Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-nitro-L-tyrosine | 50% inhibition of tyrosine incorporation at 50fold higher concentrations of nitrotyrosine | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
L-tyrosine | 37°C | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE, immunoblot | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + detyrosinated alpha-tubulin + L-tyrosine | Sus scrofa | - |
alpha-tubulin + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GST-TTL fusion protein, glutathione-sepharose affinity chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-70°C, 25 mM K+-morpholinoethanesulfonic acid, pH 6.8, 150 mM KCl, 12.5 MgCl2, 2.5 mM ATP, 1 mM dithiothreitol, 15% glycerol, at least 4 months, no loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + detyrosinated alpha-tubulin + 3-nitro-L-tyrosine | 35fold lower affinity for nitrotyrosine than for L-tyrosine, nitrotyrosine is incoorporated into the C-terminus | Sus scrofa | nitrotyrosinated alpha-tubulin + ADP + phosphate | - |
? | |
ATP + detyrosinated alpha-tubulin + L-tyrosine | - |
Sus scrofa | alpha-tubulin + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE, immunoblot | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
TTL | - |
Sus scrofa |
tubuline-tyrosine ligase | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
3-nitro-L-tyrosine | 37°C | Sus scrofa |