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Literature summary for 6.3.2.24 extracted from

  • Ueda, H.; Yoshihara, Y.; Fukushima, N.; Shiomi, H.; Nakamura, A.; Takagi, H.
    Kyotorphin (tyrosine-arginine) synthetase in rat brain synaptosomes (1987), J. Biol. Chem., 262, 8165-8173.
    View publication on PubMed
Search for more literature for 6.3.2.24

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.4-4 mM: strong Rattus norvegicus
Cu2+ 0.04-4 mM: strong Rattus norvegicus
diphosphate 10 mM: 97.6% inhibition Rattus norvegicus
K+ 50-100 mM: strong Rattus norvegicus
NH4+ 10-100 mM: strong Rattus norvegicus
phosphate 10 mM: 4.2% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0256
-
 L-tyrosine 37°C, pH 8.0 Rattus norvegicus
0.294
-
 ATP 37°C, pH 8.0 Rattus norvegicus
0.442
-
 MgCl2 37°C, pH 8.0 Rattus norvegicus
0.926
-
 L-arginine 37°C, pH 8.0 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-
synaptosome highest activity in synaptosolic fraction, minor activity membrane-bound or in solubilized fraction Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement Rattus norvegicus
Mg2+ Km: 0.442 mM, MgCl2 Rattus norvegicus
Mn2+ 102,7% of activity compared to Mg2+, may substitute for Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000 245000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + L-arginine Rattus norvegicus involved in biosynthesis of kyotorphin in rat brain L-tyrosyl-L-arginine + AMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000016
-
 37°C, pH 8.0 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + L-tyrosine + L-arginine
-
 Rattus norvegicus L-tyrosyl-L-arginine + ? 37.8% of activity compared to ATP ?
AMP + L-tyrosine + L-arginine
-
 Rattus norvegicus L-tyrosyl-L-arginine + ? 1,6% of activity compared to ATP ?
ATP + L-arginine + leucine
-
 Rattus norvegicus ? + AMP 3.5% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-arginine + lysine
-
 Rattus norvegicus ? + AMP 10.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-arginine + phenylalanine
-
 Rattus norvegicus ? + AMP 10% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-arginine + tryptophan
-
 Rattus norvegicus ? + AMP 11.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-tyrosine + glutamate
-
 Rattus norvegicus ? + AMP 26.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-tyrosine + glycine
-
 Rattus norvegicus ? + AMP 1.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-tyrosine + histidine
-
 Rattus norvegicus ? + AMP 4.0% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-tyrosine + L-arginine
-
 Rattus norvegicus L-tyrosyl-L-arginine + AMP + diphosphate i.e. kyotorphin ?
ATP + L-tyrosine + L-arginine involved in biosynthesis of kyotorphin in rat brain Rattus norvegicus L-tyrosyl-L-arginine + AMP + diphosphate
-
 ?
ATP + L-tyrosine + leucine
-
 Rattus norvegicus ? + AMP 31.4% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
ATP + L-tyrosine + lysine
-
 Rattus norvegicus ? + AMP 14.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine ?
GTP + L-tyrosine + L-arginine
-
 Rattus norvegicus L-tyrosyl-L-arginine + ? 20.2% of activity compared to ATP ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9 broad Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
8 10 28% of maximal activity at pH 8, 85% of maximal activity at pH 10 Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectric focusing
-
 6.1