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Literature summary for 6.3.2.23 extracted from

  • Dworeck, T.; Zimmermann, M.
    Site directed mutagenesis of Schizosaccharomyces pombe glutathione synthetase produces an enzyme with homoglutathione synthetase activity (2012), PLoS ONE, 7, e46580.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.23

Protein Variants

Protein Variants Comment Organism
L534A/P535A site-directed mutagenesis, the mutant shows properties similar to GSH synthetase, EC 6.3.2.3. A534 and A535 are conserved in glutathione synthetase Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine Medicago truncatula
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
 ?
ATP + gamma-L-glutamyl-L-cysteine + L-serine Medicago truncatula
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-L-serine
-
 ?
additional information Medicago truncatula hGSH synthetase found in plants is much more specific for beta-Ala, although it also accepts Gly as a substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago truncatula
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Medicago truncatula ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
 ?
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Medicago truncatula ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homo-glutathione ?
ATP + gamma-L-glutamyl-L-cysteine + L-serine
-
 Medicago truncatula ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-L-serine
-
 ?
ATP + gamma-L-glutamyl-L-cysteine + L-serine
-
 Medicago truncatula ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-L-serine i.e. hydroxymethyl-glutathione ?
additional information hGSH synthetase found in plants is much more specific for beta-Ala, although it also accepts Gly as a substrate Medicago truncatula ?
-
 ?

Synonyms

Synonyms Comment Organism
hGSH synthetase
-
 Medicago truncatula
hGSH-synthetase
-
 Medicago truncatula
homoglutathione synthetase
-
 Medicago truncatula

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Medicago truncatula

General Information

General Information Comment Organism
evolution homoglutathione synthetase has evolved from glutathione synthetase, EC 6.3.2.3, by a single gene duplication event Medicago truncatula