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Literature summary for 6.3.2.23 extracted from

  • Galant, A.; Arkus, K.A.; Zubieta, C.; Cahoon, R.E.; Jez, J.M.
    Structural basis for evolution of product diversity in soybean glutathione biosynthesis (2009), Plant Cell, 21, 3450-3458.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Glycine max

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method Glycine max

Protein Variants

Protein Variants Comment Organism
L487A improved catalytic efficiency using glcyine Glycine max
L487A/P488A conversion of substrate preference from beta-alanine to glycine Glycine max
P488A improved catalytic efficiency using glcyine Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information glycine, value above 100 mM, wild-type enzyme Glycine max
0.023
-
ATP
-
Glycine max
0.044
-
gamma-L-glutamyl-L-cysteine
-
Glycine max
2.2
-
glycine mutant L487A/P488A Glycine max
3.39
-
beta-Alanine wild-type enzyme Glycine max
3.8
-
beta-Alanine mutant L487A Glycine max
5.1
-
glycine mutant P488A Glycine max
6.5
-
glycine mutant L487A Glycine max
8
-
beta-Alanine mutant P488A Glycine max
24.8
-
beta-Alanine mutant L487A/P488A Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mixed benzamidine-sepharose/Ni2+-NTA column, Superdex-200 FPLC column Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine assay at pH 7.5, 25°C Glycine max ?
-
?
ATP + gamma-L-glutamyl-L-cysteine + glycine assay at pH 7.5, 25°C Glycine max ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Glycine max

Synonyms

Synonyms Comment Organism
hGS
-
Glycine max
homoglutathione synthetase
-
Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
glycine wild-type enzyme Glycine max
0.046
-
glycine mutant L487A Glycine max
0.077
-
beta-Alanine mutant L487A/P488A Glycine max
0.211
-
beta-Alanine mutant L487A Glycine max
0.274
-
glycine mutant P488A Glycine max
0.313
-
beta-Alanine mutant P488A Glycine max
0.708
-
beta-Alanine wild-type enzyme Glycine max
0.95
-
glycine mutant L487A/P488A Glycine max
56.82
-
gamma-L-glutamyl-L-cysteine
-
Glycine max
73.91
-
ATP
-
Glycine max