Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Glycine max |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
L487A | improved catalytic efficiency using glcyine | Glycine max |
L487A/P488A | conversion of substrate preference from beta-alanine to glycine | Glycine max |
P488A | improved catalytic efficiency using glcyine | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | glycine, value above 100 mM, wild-type enzyme | Glycine max | |
0.023 | - |
ATP | - |
Glycine max | |
0.044 | - |
gamma-L-glutamyl-L-cysteine | - |
Glycine max | |
2.2 | - |
glycine | mutant L487A/P488A | Glycine max | |
3.39 | - |
beta-Alanine | wild-type enzyme | Glycine max | |
3.8 | - |
beta-Alanine | mutant L487A | Glycine max | |
5.1 | - |
glycine | mutant P488A | Glycine max | |
6.5 | - |
glycine | mutant L487A | Glycine max | |
8 | - |
beta-Alanine | mutant P488A | Glycine max | |
24.8 | - |
beta-Alanine | mutant L487A/P488A | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mixed benzamidine-sepharose/Ni2+-NTA column, Superdex-200 FPLC column | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | assay at pH 7.5, 25°C | Glycine max | ? | - |
? | |
ATP + gamma-L-glutamyl-L-cysteine + glycine | assay at pH 7.5, 25°C | Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Glycine max |
Synonyms | Comment | Organism |
---|---|---|
hGS | - |
Glycine max |
homoglutathione synthetase | - |
Glycine max |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
glycine | wild-type enzyme | Glycine max | |
0.046 | - |
glycine | mutant L487A | Glycine max | |
0.077 | - |
beta-Alanine | mutant L487A/P488A | Glycine max | |
0.211 | - |
beta-Alanine | mutant L487A | Glycine max | |
0.274 | - |
glycine | mutant P488A | Glycine max | |
0.313 | - |
beta-Alanine | mutant P488A | Glycine max | |
0.708 | - |
beta-Alanine | wild-type enzyme | Glycine max | |
0.95 | - |
glycine | mutant L487A/P488A | Glycine max | |
56.82 | - |
gamma-L-glutamyl-L-cysteine | - |
Glycine max | |
73.91 | - |
ATP | - |
Glycine max |