Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-hydroxy-2-nonenal | 4-hydroxy-2-nonenal causes a rapid dose- and time-dependent increase in A549 cell GCL activity. Maximal activation of GCL occurs at 30 min in response to 50 microM 4-hydroxy-2-nonenal | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
both regulatory subunit GCLM and catalytic subunit GCLC | Mus musculus |
both regulatory subunit GCLM and catalytic subunit GCLC | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxy-2-nonenal | treatment with 4-hydroxy-2-nonenal results in the dose-dependent adduction of both monomeric GCLC and GCLM. 4-Hydroxy-2-nonenal-mediated adduction of monomeric GCLC results in a dose-dependent increase in GCLC enzymatic activity. Treatment of GCL holoenzyme causes a dose-dependent decrease in GCL activity. 4-Hydroxy-2-nonenal-mediated inhibition of GCL holoenzyme activity is associated with a reduction in the levels of heterodimeric GCL holoenzyme complex due to increase in high molecular weight complexes. 4-Hydroxy-2-nonenal modification simultaneously activates monomeric GCLC activity and prevents its ability to heterodimerize with GCLM and form functional GCL holoenzyme | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | 4-hydroxy-2-nonenal alters GCL holoenzyme formation and activity via direct posttranslational modification of the GCL subunits in vitro. 4-Hydroxy-2-nonenal directly modifies Cys553 of catalytic subunit GCLC and Cys35 of modulatory subunit GCLM in vitro, which significantly increases monomeric GCLC enzymatic activity, but reduces GCL holoenzyme activity and formation of the GCL holoenzymecomplex | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
GCL | - |
Mus musculus |
GCLC | - |
Mus musculus |
GCLM | - |
Mus musculus |