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Literature summary for 6.3.2.2 extracted from

  • Backos, D.S.; Brocker, C.N.; Franklin, C.C.
    Manipulation of cellular GSH biosynthetic capacity via TAT-mediated protein transduction of wild-type or a dominant-negative mutant of glutamate cysteine ligase alters cell sensitivity to oxidant-induced cytotoxicity (2010), Toxicol. Appl. Pharmacol., 243, 35-45.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology a protein transduction approach whereby recombinant GCL protein can be rapidly and directly transferred into cells when coupled to the HIV TAT protein transduction domain. The TAT-GCL fusion proteins are capable of heterodimerization and formation of functional GCL holoenzyme in vitro. Exposure of Hepa-1c1c7 cells to the TAT-GCL fusion proteins results in the time- and dose-dependent transduction of both GCL subunits and increased cellular GCL activity and glutathione levels. A heterodimerization-competent, enzymatically deficient GCLC-TAT mutant was also generated in an attempt to create a dominant-negative suppressor of GCL Mus musculus

Protein Variants

Protein Variants Comment Organism
E103A transduction of Hepa-1c1c7 cells with a catalytically inactive GCL catalytic subunit E103A mutant decreases cellular GCL activity in a dose-dependent manner Mus musculus
additional information a protein transduction approach whereby recombinant GCL protein can be rapidly and directly transferred into cells when coupled to the HIV TAT protein transduction domain. The TAT-GCL fusion proteins are capable of heterodimerization and formation of functional GCL holoenzyme in vitro. Exposure of Hepa-1c1c7 cells to the TAT-GCL fusion proteins results in the time- and dose-dependent transduction of both GCL subunits and increased cellular GCL activity and glutathione levels. A heterodimerization-competent, enzymatically deficient GCLC-TAT mutant was also generated in an attempt to create a dominant-negative suppressor of GCL Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
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