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Literature summary for 6.3.2.2 extracted from

  • Liedschulte, V.; Wachter, A.; Zhigang, A.; Rausch, T.
    Exploiting plants for glutathione (GSH) production: Uncoupling GSH synthesis from cellular controls results in unprecedented GSH accumulation (2010), Plant Biotechnol. J., 8, 1-14.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, transformation into tobacco plants Streptococcus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
ATP
-
Streptococcus thermophilus
0.399
-
L-cysteine
-
Streptococcus thermophilus
33
-
L-glutamate
-
Streptococcus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Streptococcus thermophilus 5829
-
plastid
-
Streptococcus thermophilus 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
SDS-PAGE Streptococcus thermophilus

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus D7P1H2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity chromatography Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + L-cysteine + ATP
-
Streptococcus thermophilus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
gamma-glutamylcysteine ligase bifunctional enzyme, also glutathione synthetase activity, EC 6.3.2.3 Streptococcus thermophilus
StGCL-GS
-
Streptococcus thermophilus