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Literature summary for 6.3.2.2 extracted from

  • Miyake, K.; Kakita, S.
    A novel catalytic ability of gamma-glutamylcysteine synthetase of Escherichia coli and its application in theanine production (2009), Biosci. Biotechnol. Biochem., 73, 2677-2683.
    View publication on PubMed

Application

Application Comment Organism
synthesis resting cells of Escherichia coli expressing gamma-glutamylcysteine synthetase, with ATP regeneration through glycolysis, synthesize 12.1 mM theanine in 18 h from 429 mM ethylamine Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + butylamine
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Escherichia coli ADP + phosphate + N-butyl-L-glutamine
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?
ATP + L-Glu + ethylamine
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Escherichia coli ADP + phosphate + N-ethyl-L-glutamine
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?
ATP + L-Glu + methylamine
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Escherichia coli ADP + phosphate + N-methyl-L-glutamine
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?
ATP + L-Glu + n-propylamine
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Escherichia coli ADP + phosphate + N-propyl-L-glutamine
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?
additional information enzyme is able to to combine glutamine and amines to form gamma-glutamylamides. The reaction rate depende on the length if the methylene chain of the amines in the following decreasing order: n-propylamine > butylamine > ethylamine > methylamine Escherichia coli ?
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