Cloned (Comment) | Organism |
---|---|
partial DNA and amino acid sequence determination and analysis | Unio tumidus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Unio tumidus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Unio tumidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | Unio tumidus | first step in the biosynthesis of glutathione, pathway overview | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Unio tumidus | B4YE15 | fragment | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive gland | - |
Unio tumidus | - |
gill | - |
Unio tumidus | - |
additional information | seasonal variations of activity in the digestive gland and to a lesser extent in the gills occur with activity increasing in spring compared to winter, no sex differences | Unio tumidus | - |
Storage Stability | Organism |
---|---|
-80°C, gills and in the digestive gland frozen without buffer, at least 6 days, loss of activity compared to fresh tissues, the activity in digestive gland, but in gills, is stable after addition of mM phosphate buffer containing PMSF, L-serine borate, and acivicin | Unio tumidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | - |
Unio tumidus | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | first step in the biosynthesis of glutathione, pathway overview | Unio tumidus | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCL | - |
Unio tumidus |
glutamate cysteine ligase | - |
Unio tumidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Unio tumidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.3 | assay at | Unio tumidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Unio tumidus |