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Literature summary for 6.3.2.2 extracted from

  • Coffinet, S.; Cossu-Leguille, C.; Rodius, F.; Vasseur, P.
    Glutamate cysteine ligase (GCL) in the freshwater bivalve Unio tumidus: Impact of storage conditions and seasons on activity and identification of partial coding sequence of the catalytic subunit (2008), Comp. Biochem. Physiol. B, 151B, 88-95.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
partial DNA and amino acid sequence determination and analysis Unio tumidus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Unio tumidus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Unio tumidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Unio tumidus first step in the biosynthesis of glutathione, pathway overview ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Unio tumidus B4YE15 fragment
-

Source Tissue

Source Tissue Comment Organism Textmining
digestive gland
-
Unio tumidus
-
gill
-
Unio tumidus
-
additional information seasonal variations of activity in the digestive gland and to a lesser extent in the gills occur with activity increasing in spring compared to winter, no sex differences Unio tumidus
-

Storage Stability

Storage Stability Organism
-80°C, gills and in the digestive gland frozen without buffer, at least 6 days, loss of activity compared to fresh tissues, the activity in digestive gland, but in gills, is stable after addition of mM phosphate buffer containing PMSF, L-serine borate, and acivicin Unio tumidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
Unio tumidus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?
ATP + L-glutamate + L-cysteine first step in the biosynthesis of glutathione, pathway overview Unio tumidus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
GCL
-
Unio tumidus
glutamate cysteine ligase
-
Unio tumidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Unio tumidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.3 assay at Unio tumidus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Unio tumidus