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Literature summary for 6.3.2.2 extracted from

  • Wheeler, G.L.; Quinn, K.A.; Perrone, G.; Dawes, I.W.; Grant, C.M.
    Glutathione regulates the expression of gamma-glutamylcysteine synthetase via the Met4 transcription factor (2002), Mol. Microbiol., 46, 545-556.
    View publication on PubMed
Search for more literature for 6.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
mcCDC34 an ubiquitine conjugated protein, induces gamma-glutamylcysteine synthetase expression only in glutathione synthetase-dficient mutants, not in the wild-type Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme at high level in deletion mutant strains of GHS1 and GSH2, the latter encoding the glutathione synthetase, the GSH1 promotor is Met4-inducible and GSH repressible Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information mutants with a deletion of GSH1 cannot grow, a high level expression from a plasmid of the enzyme can compensate for adeletion of gene GSH2 encoding glutathione synthatase, the enzyme catalyzing the second step of glutathione biosynthesis Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
GSH regulatory function, represses expression of GSH1 gene Saccharomyces cerevisiae
methionine inhibits induction of GSH1 expression, independently of GSH Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Glu + L-Cys Saccharomyces cerevisiae
-
 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
additional information Saccharomyces cerevisiae Met4 regulates the GSH1 expression in response to GSH availability, model for genetic regulation and control of GSH biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 gene GSH1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + L-Cys
-
 Saccharomyces cerevisiae ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
additional information Met4 regulates the GSH1 expression in response to GSH availability, model for genetic regulation and control of GSH biosynthesis Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
gamma-Glutamylcysteine synthetase
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae