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Literature summary for 6.3.2.2 extracted from

  • Hussein, A.S.; Walter, R.D.
    Purification and characterization of gamma-glutamylcysteine synthetase from Ascaris suum (1995), Mol. Biochem. Parasitol., 72, 57-64.
    View publication on PubMed
Search for more literature for 6.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
buthionine sulfoximine
-
 Ascaris suum
cystamine
-
 Ascaris suum
glutathione
-
 Ascaris suum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 L-aminobutanoate
-
 Ascaris suum
0.41
-
 L-Cys
-
 Ascaris suum
0.94
-
 L-Glu
-
 Ascaris suum
1.41
-
 ATP
-
 Ascaris suum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration, a 100000 MW enzyme form is also detected Ascaris suum
100000
-
 gel filtration, a 70000 MW enzyme form is also detected Ascaris suum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Glu + L-Cys Ascaris suum rate-limiting step in glutathione biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ascaris suum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ascaris suum

Storage Stability

Storage Stability Organism
4°C or -20°C, about 50% loss of activity after 7 days and total loss of activity after 1 month Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + L-2-aminobutanoate
-
 Ascaris suum ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-Cys
-
 Ascaris suum ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys rate-limiting step in glutathione biosynthesis Ascaris suum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Ascaris suum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Ascaris suum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, stable Ascaris suum
45
-
 5 min, 70% loss of activity Ascaris suum
56
-
 total loss of activity Ascaris suum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Ascaris suum