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Literature summary for 6.3.2.2 extracted from

  • Davis, J.S.; Balinsky, J.B.; Harington, J.S.; Shepherd, J.B.
    Assay, purification, properties and mechanism of action of gamma-glutamylcysteine synthetase from the liver of the rat and Xenopus laevis (1973), Biochem. J., 133, 667-678.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Gly stimulates Rattus norvegicus
Gly no effect Xenopus laevis

Inhibitors

Inhibitors Comment Organism Structure
glutathione inhibited by both GSSG and GSH Rattus norvegicus
glutathione GSSG inhibits, GSH has no inhibitory effect Xenopus laevis
K+
-
Rattus norvegicus
K+
-
Xenopus laevis
NAD+
-
Rattus norvegicus
NAD+
-
Xenopus laevis
NADH
-
Rattus norvegicus
NADH
-
Xenopus laevis
NADP+
-
Rattus norvegicus
NADP+
-
Xenopus laevis
NADPH
-
Rattus norvegicus
NADPH
-
Xenopus laevis

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
-
Xenopus laevis

Reaction

Reaction Comment Organism Reaction ID
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine bi bi uni uni ping pong mechanism, with glutamyl-enzyme as intermediate before the addition of cys and the release of gamma-glutamylcysteine Xenopus laevis
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine tri tri sequential mechanism Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus
additional information
-
-
Xenopus laevis

Storage Stability

Storage Stability Organism
4°C, or in the frozen state, 25-30% loss of activity Xenopus laevis
4°C, or in the frozen state, stable for 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + L-Cys
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
?
ATP + L-Glu + L-Cys
-
Xenopus laevis ADP + phosphate + gamma-L-Glu-L-Cys
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
Rattus norvegicus
7.7
-
-
Xenopus laevis