Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Gly | stimulates | Rattus norvegicus | |
Gly | no effect | Xenopus laevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | inhibited by both GSSG and GSH | Rattus norvegicus | |
glutathione | GSSG inhibits, GSH has no inhibitory effect | Xenopus laevis | |
K+ | - |
Rattus norvegicus | |
K+ | - |
Xenopus laevis | |
NAD+ | - |
Rattus norvegicus | |
NAD+ | - |
Xenopus laevis | |
NADH | - |
Rattus norvegicus | |
NADH | - |
Xenopus laevis | |
NADP+ | - |
Rattus norvegicus | |
NADP+ | - |
Xenopus laevis | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Xenopus laevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Xenopus laevis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine | bi bi uni uni ping pong mechanism, with glutamyl-enzyme as intermediate before the addition of cys and the release of gamma-glutamylcysteine | Xenopus laevis | |
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine | tri tri sequential mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
- |
Xenopus laevis |
Storage Stability | Organism |
---|---|
4°C, or in the frozen state, 25-30% loss of activity | Xenopus laevis |
4°C, or in the frozen state, stable for 2 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | - |
Xenopus laevis | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Rattus norvegicus |
7.7 | - |
- |
Xenopus laevis |