Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.2 extracted from

  • Seelig, G.F.; Meister, A.
    Glutathione biosynthesis: gamma-Glutamylcysteine synthetase from rat kidney (1985), Methods Enzymol., 113, 379-390.
    View publication on PubMed

General Stability

General Stability Organism
treatment with 50 mM DTT for more than 1 h at 4°C, leads to some loss of activity, which is partially reversible. Further treatment leads to irreversible loss of activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
beta-Methylglutamate
-
Rattus norvegicus
buthionine sulfoximine
-
Rattus norvegicus
cystamine completely reversible by DTT Rattus norvegicus
D-3-amino-1-chloro-2-pentanone highly potent irreversible inactivator Rattus norvegicus
D-Glu
-
Rattus norvegicus
gamma-Methylglutamate
-
Rattus norvegicus
glutathione
-
Rattus norvegicus
L-2-Amino-4-oxo-5-chloropentanoate inactivation requires very low concentration, 0.003-0.006 mM, of Mg2+ or certain other divalent cations, L-Glu, but not D-Glu protects competitively against inactivation, protection is increased in the presence of ATP or ADP Rattus norvegicus
L-3-Amino-1-chloro-2-pentanone highly potent irreversible inactivator Rattus norvegicus
methionine sulfoximine
-
Rattus norvegicus
Prothionine sulfoximine
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
L-Cys ATP, holoenzyme and recombinant heavy subunit Rattus norvegicus
0.3
-
L-Cys
-
Rattus norvegicus
1
-
L-2-aminobutanoate
-
Rattus norvegicus
1.6
-
L-Glu
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Glu + L-Cys Rattus norvegicus glutathione biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-methylglutamate + L-Cys i.e. 2-amino-2-methylpentanedioate Rattus norvegicus ADP + phosphate + alpha-methylglutamyl-L-Cys
-
?
ATP + beta-aminoglutarate + L-Cys i.e. 3-aminopentanedioate Rattus norvegicus ADP + phosphate + beta-aminoglutaryl-L-Cys
-
?
ATP + beta-methylglutamate + L-Cys i.e. 2-amino-3-methylpentanedioate Rattus norvegicus ADP + phosphate + beta-methylglutamyl-L-Cys
-
?
ATP + D-Glu + L-Cys
-
Rattus norvegicus ADP + phosphate + D-Glu-L-Cys
-
?
ATP + L-Glu + beta-chloro-L-Ala
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala
-
?
ATP + L-Glu + DL-allylglycine
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-DL-allylglycine
-
?
ATP + L-Glu + hydroxylamine slow reaction rate Rattus norvegicus ADP + phosphate + gamma-L-Glu-hydroxylamine
-
?
ATP + L-Glu + L-2-aminobutanoate
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
?
ATP + L-Glu + L-Ala
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Ala
-
?
ATP + L-Glu + L-Cys
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
?
ATP + L-Glu + L-Cys glutathione biosynthesis Rattus norvegicus ?
-
?
ATP + L-Glu + L-homocysteine
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-homocysteine
-
?
ATP + L-Glu + L-norvaline
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-norvaline
-
?
ATP + L-Glu + L-Thr
-
Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Thr
-
?
ATP + N-methyl-L-glutarate + L-Cys
-
Rattus norvegicus ADP + phosphate + N-methyl-L-glutaryl-L-Cys
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.4
-
Rattus norvegicus