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Literature summary for 6.3.2.2 extracted from

  • Watanabe, K.; Murata, K.; Kimura, A.
    Purification and characterization of gamma-glutamylcysteine synthetase of Escherichia coli B (1986), Agric. Biol. Chem., 50, 1925-1930.
No PubMed abstract available
Search for more literature for 6.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
cystamine
-
 Escherichia coli
cysteamine
-
 Escherichia coli
glutathione feed-back inhibition; GSH inhibits, GSSG has no inhibitory effect Escherichia coli
Hg2+
-
 Escherichia coli
iodoacetamide
-
 Escherichia coli
NEM
-
 Escherichia coli
PCMB
-
 Escherichia coli
Thiocholine disulfide
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 ATP
-
 Escherichia coli
0.09
-
 L-Cys
-
 Escherichia coli
0.5
-
 L-Glu
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent metal ion required, especially Mg2+, optimal concentration depending on ATP concentration Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + D-Cys
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-D-Cys
-
 ?
ATP + L-Glu + D-Cys
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-D-Cys
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-2-aminobutanoate
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
ATP + L-Glu + L-Cys
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 pH 7.0, 35 min, 50% loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 55°C, 35 min, 50% loss of activity Escherichia coli