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Literature summary for 6.3.2.17 extracted from

  • Zhao, R.; Titus, S.; Gao, F.; Moran, R.G.; Goldman, I.D.
    Molecular analysis of murine leukemia cell lines resistant to 5,10-dideazatetrahydrofolate identifies several amino acids critical to the function of folylpolyglutamate synthetase (2000), J. Biol. Chem., 275, 26599-26606.
    View publication on PubMed
Search for more literature for 6.3.2.17

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild type and mutant enzymes in AUXB1 cell line, which does not express measurable enzyme activity and is auxotrophic for the products of folate metabolism Mus musculus

Protein Variants

Protein Variants Comment Organism
A337V L44 cells Mus musculus
G178E L7 cells Mus musculus
G320D L15 cells Mus musculus
S180F L51 cells Mus musculus
S67F L7 cells Mus musculus
S67F L51 cells Mus musculus
T339I L44 cells Mus musculus
W445stop L15 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
5,10-dideaza-5,6,7,8-tetrahydrofolic acid lometrexo, used to generate the 5,10-dideazatetrahydrofolate resistant cells sublines Mus musculus
methotrexate the level required to inhibit the 5,10-dideazatetrahydrofolate resistant cell sublines is unchanged or slightly decreased compared with wild type cells Mus musculus
N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid raltitrexed, no inhibition observed with the 5,10-dideazatetrahydrofolate resistant cells sublines Mus musculus
pemetrexed disodium ALIMTA, no inhibition observed with the 5,10-dideazatetrahydrofolate resistant cells sublines Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 mouse
-

Source Tissue

Source Tissue Comment Organism Textmining
L-1210 cell leukemia line, wild type and L7, L15, L44 and L51 5,10-dideazatetrahydrofolate resistant cells sublines Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000006
-
 enzyme from L44 cells Mus musculus
0.0000001
-
 enzyme from L7 cells Mus musculus
0.0000001
-
 enzyme from L15 cells Mus musculus
0.0000001
-
 enzyme from L51 cells Mus musculus
0.000008
-
 wild type enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
-
 Mus musculus ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
 ?
ATP + 5-formyl-5,6,7,8-tetrahydrofolate + L-glutamate
-
 Mus musculus ADP + 5-formyl-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
 ?
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate folic acid Mus musculus ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
-
 ?