Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.17 extracted from

  • Sanghani, S.P.; Sanghani, P.C.; Moran, R.G.
    Identification of three key active site residues in the C-terminal domain of human recombinant folylpoly-gamma-glutamate synthetase by site-directed mutagenesis (1999), J. Biol. Chem., 274, 27018-27027.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild type and mutant enzymes in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C346A activity comparable with that of wild type enzyme Homo sapiens
D335A with no significant activity Homo sapiens
D376A only slightly lower activity than the wild type enzyme Homo sapiens
D378A only slightly lower activity than the wild type enzyme Homo sapiens
H338A only 0.3% activity of the wild type enzyme Homo sapiens
K384A only slightly lower activity than the wild type enzyme Homo sapiens
R377A with no significant activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 2 mM, 30 to 85% loss of activity in 5 min, depending on the enzyme concentration Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
ATP pH 8.9, 37ÂșC, HPLC assay, R377A mutant Homo sapiens
0.0059
-
aminopterin pH 8.9, 37ÂșC, HPLC assay, R377A mutant Homo sapiens
0.0065
-
aminopterin pH 8.9, 37ÂșC, HPLC assay, H338A mutant Homo sapiens
0.009
-
aminopterin pH 8.9, 37ÂșC, charcoal assay, wild type recombinant enzyme Homo sapiens
0.011
-
aminopterin pH 8.9, 37ÂșC, charcoal assay, K384A mutant Homo sapiens
0.013
-
aminopterin pH 8.9, 37ÂșC, charcoal assay, C346A mutant Homo sapiens
0.016
-
ATP pH 8.9, 37ÂșC, HPLC assay, H338A mutant Homo sapiens
0.017
-
aminopterin pH 8.9, 37ÂșC, charcoal assay, D378A mutant Homo sapiens
0.028
-
ATP pH 8.9, 37ÂșC, charcoal assay, D376A mutant Homo sapiens
0.031
-
aminopterin pH 8.9, 37ÂșC, charcoal assay, D376A mutant Homo sapiens
0.04
-
ATP pH 8.9, 37ÂșC, charcoal assay, C346A mutant Homo sapiens
0.045
-
ATP pH 8.9, 37ÂșC, charcoal assay, K384A mutant Homo sapiens
0.046
-
ATP pH 8.9, 37ÂșC, charcoal assay, wild type recombinant enzyme Homo sapiens
0.06
-
ATP pH 8.9, 37ÂșC, charcoal assay, D378A mutant Homo sapiens
0.18
-
aminopterin pH 8.9, 37ÂșC, HPLC assay, D335A mutant Homo sapiens
0.235
-
glutamic acid pH 8.9, 37ÂșC, charcoal assay, wild type recombinant enzyme Homo sapiens
0.267
-
ATP pH 8.9, 37ÂșC, HPLC assay, D335A mutant Homo sapiens
0.43
-
L-Glutamic acid pH 8.9, 37ÂșC, charcoal assay, K384A mutant Homo sapiens
0.52
-
L-Glutamic acid pH 8.9, 37ÂșC, charcoal assay, C346A mutant Homo sapiens
0.83
-
L-Glutamic acid pH 8.9, 37ÂșC, charcoal assay, D378A mutant Homo sapiens
2.9
-
L-Glutamic acid pH 8.9, 37ÂșC, charcoal assay, D376A mutant Homo sapiens
91
-
L-Glutamic acid pH 8.9, 37ÂșC, HPLC assay, D335A mutant Homo sapiens
140
-
L-Glutamic acid pH 8.9, 37ÂșC, HPLC assay, H338A mutant Homo sapiens
350
-
L-Glutamic acid pH 8.9, 37ÂșC, HPLC assay, R377A mutant Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, chromatography on Sephacryl S-100 and DEAE-Sephacel columns Homo sapiens

Storage Stability

Storage Stability Organism
-25ÂșC, 50% glycerol, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + aminopterin + L-glutamate
-
Homo sapiens ?
-
?