Cloned (Comment) | Organism |
---|---|
expression of wild type and mutant enzymes in Sf9 insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C346A | activity comparable with that of wild type enzyme | Homo sapiens |
D335A | with no significant activity | Homo sapiens |
D376A | only slightly lower activity than the wild type enzyme | Homo sapiens |
D378A | only slightly lower activity than the wild type enzyme | Homo sapiens |
H338A | only 0.3% activity of the wild type enzyme | Homo sapiens |
K384A | only slightly lower activity than the wild type enzyme | Homo sapiens |
R377A | with no significant activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 2 mM, 30 to 85% loss of activity in 5 min, depending on the enzyme concentration | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
ATP | pH 8.9, 37ÂșC, HPLC assay, R377A mutant | Homo sapiens | |
0.0059 | - |
aminopterin | pH 8.9, 37ÂșC, HPLC assay, R377A mutant | Homo sapiens | |
0.0065 | - |
aminopterin | pH 8.9, 37ÂșC, HPLC assay, H338A mutant | Homo sapiens | |
0.009 | - |
aminopterin | pH 8.9, 37ÂșC, charcoal assay, wild type recombinant enzyme | Homo sapiens | |
0.011 | - |
aminopterin | pH 8.9, 37ÂșC, charcoal assay, K384A mutant | Homo sapiens | |
0.013 | - |
aminopterin | pH 8.9, 37ÂșC, charcoal assay, C346A mutant | Homo sapiens | |
0.016 | - |
ATP | pH 8.9, 37ÂșC, HPLC assay, H338A mutant | Homo sapiens | |
0.017 | - |
aminopterin | pH 8.9, 37ÂșC, charcoal assay, D378A mutant | Homo sapiens | |
0.028 | - |
ATP | pH 8.9, 37ÂșC, charcoal assay, D376A mutant | Homo sapiens | |
0.031 | - |
aminopterin | pH 8.9, 37ÂșC, charcoal assay, D376A mutant | Homo sapiens | |
0.04 | - |
ATP | pH 8.9, 37ÂșC, charcoal assay, C346A mutant | Homo sapiens | |
0.045 | - |
ATP | pH 8.9, 37ÂșC, charcoal assay, K384A mutant | Homo sapiens | |
0.046 | - |
ATP | pH 8.9, 37ÂșC, charcoal assay, wild type recombinant enzyme | Homo sapiens | |
0.06 | - |
ATP | pH 8.9, 37ÂșC, charcoal assay, D378A mutant | Homo sapiens | |
0.18 | - |
aminopterin | pH 8.9, 37ÂșC, HPLC assay, D335A mutant | Homo sapiens | |
0.235 | - |
glutamic acid | pH 8.9, 37ÂșC, charcoal assay, wild type recombinant enzyme | Homo sapiens | |
0.267 | - |
ATP | pH 8.9, 37ÂșC, HPLC assay, D335A mutant | Homo sapiens | |
0.43 | - |
L-Glutamic acid | pH 8.9, 37ÂșC, charcoal assay, K384A mutant | Homo sapiens | |
0.52 | - |
L-Glutamic acid | pH 8.9, 37ÂșC, charcoal assay, C346A mutant | Homo sapiens | |
0.83 | - |
L-Glutamic acid | pH 8.9, 37ÂșC, charcoal assay, D378A mutant | Homo sapiens | |
2.9 | - |
L-Glutamic acid | pH 8.9, 37ÂșC, charcoal assay, D376A mutant | Homo sapiens | |
91 | - |
L-Glutamic acid | pH 8.9, 37ÂșC, HPLC assay, D335A mutant | Homo sapiens | |
140 | - |
L-Glutamic acid | pH 8.9, 37ÂșC, HPLC assay, H338A mutant | Homo sapiens | |
350 | - |
L-Glutamic acid | pH 8.9, 37ÂșC, HPLC assay, R377A mutant | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, chromatography on Sephacryl S-100 and DEAE-Sephacel columns | Homo sapiens |
Storage Stability | Organism |
---|---|
-25ÂșC, 50% glycerol, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + aminopterin + L-glutamate | - |
Homo sapiens | ? | - |
? |