Any feedback?
Literature summary for 6.3.2.17 extracted from
- Mouse folylpoly-gamma-glutamate synthetase isoforms respond differently to feedback inhibition by folylpolyglutamate cofactors (2002), Biochemistry, 41, 226-235.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the liver or L1210 isoforms in Sf9 insect cells | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus |  |
| (6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
| (6S)-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
| (6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
| (6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
5,10-dideazatetrahydrofolic acid | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
| 0.0014 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
| 0.002 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
| 0.0029 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus | |
| 0.0063 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus | |
| 0.0076 | - |
5,10-dideazatetrahydrofolic acid | pH 8.9, 37ºC, liver isoform | Mus musculus | |
| 0.0154 | - |
aminopterin | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
| 0.0177 | - |
aminopterin | pH 8.9, 37ºC, liver isoform | Mus musculus | |
| 0.054 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
| 0.064 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus | |
| 0.075 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
| 0.122 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60600 | - |
L1210 isoform | Mus musculus |
| 62000 | - |
liver isoform | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + tetrahydrofolate-Glu + L-glutamate | Mus musculus | - |
ADP + phosphate + tetrahydrofolyl-Glu2 | - |
? | |
| additional information | Mus musculus | the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
mouse | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, gel filtration and ion-exchange chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| L-1210 cell | leukemia line | Mus musculus | - |
| liver | - |
Mus musculus | - |
| additional information | the isozymes from the two tissues differ by 18 amino acids at their amino terminus | Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20ºC, buffered 50% glycerol, quite stable | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + (6R)-10-formyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
| ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
| ATP + (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | poor substrate | Mus musculus | ADP + phosphate + (6S)-5-formyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
| ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | poor substrate | Mus musculus | ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
| ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyldiglutamate | - |
? | |
| ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate | - |
Mus musculus | ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate | - |
? | |
| ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
| ATP + aminopterin + L-glutamate | - |
Mus musculus | ? | - |
? | |
| ATP + tetrahydrofolate-Glu + L-glutamate | - |
Mus musculus | ADP + phosphate + tetrahydrofolyl-Glu2 | - |
? | |
| additional information | alterations in the amino terminal structure of the enzyme may influence its catalytic and/or substrate binding properties | Mus musculus | ? | - |
? | |
| additional information | the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell | Mus musculus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
5,10-dideazatetrahydrofolic acid | L1210 isoform | Mus musculus | |
| 0.073 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
| 0.095 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
| 0.11 | - |
5-methyl-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
| 0.115 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus | |
| 0.116 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
| 0.145 | - |
5,10-dideazatetrahydrofolic acid | liver isoform | Mus musculus | |
| 0.165 | - |
aminopterin | L1210 isoform | Mus musculus | |
| 0.212 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus | |
| 0.215 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus | |
| 0.22 | - |
aminopterin | liver isoform | Mus musculus | |
| 0.22 | - |
5-methyl-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
| 0.0023 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
| 0.0024 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
| 0.0037 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
| 0.0042 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, L1210 isoform | Mus musculus | |
| 0.0052 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, liver isoform | Mus musculus | |
| 0.0083 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, L1210 isoform | Mus musculus | |
| 0.0083 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, L1210 isoform | Mus musculus | |
| 0.02 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
| 0.021 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
| 0.022 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
| 0.043 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, liver isoform | Mus musculus | |
| 0.054 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, liver isoform | Mus musculus | |
| 0.055 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
| 0.066 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
| 0.084 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
