Cloned (Comment) | Organism |
---|---|
expression of the liver or L1210 isoforms in Sf9 insect cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus | |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
5,10-dideazatetrahydrofolic acid | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
0.0014 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
0.002 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
0.0029 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus | |
0.0063 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus | |
0.0076 | - |
5,10-dideazatetrahydrofolic acid | pH 8.9, 37ºC, liver isoform | Mus musculus | |
0.0154 | - |
aminopterin | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
0.0177 | - |
aminopterin | pH 8.9, 37ºC, liver isoform | Mus musculus | |
0.054 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
0.064 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus | |
0.075 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, L1210 isoform | Mus musculus | |
0.122 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | pH 8.9, 37ºC, liver isoform | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60600 | - |
L1210 isoform | Mus musculus |
62000 | - |
liver isoform | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tetrahydrofolate-Glu + L-glutamate | Mus musculus | - |
ADP + phosphate + tetrahydrofolyl-Glu2 | - |
? | |
additional information | Mus musculus | the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
mouse | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, gel filtration and ion-exchange chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
L-1210 cell | leukemia line | Mus musculus | - |
liver | - |
Mus musculus | - |
additional information | the isozymes from the two tissues differ by 18 amino acids at their amino terminus | Mus musculus | - |
Storage Stability | Organism |
---|---|
-20ºC, buffered 50% glycerol, quite stable | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + (6R)-10-formyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
ATP + (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | poor substrate | Mus musculus | ADP + phosphate + (6S)-5-formyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | poor substrate | Mus musculus | ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyldiglutamate | - |
? | |
ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate | - |
Mus musculus | ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate | - |
? | |
ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate | - |
Mus musculus | ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
ATP + aminopterin + L-glutamate | - |
Mus musculus | ? | - |
? | |
ATP + tetrahydrofolate-Glu + L-glutamate | - |
Mus musculus | ADP + phosphate + tetrahydrofolyl-Glu2 | - |
? | |
additional information | alterations in the amino terminal structure of the enzyme may influence its catalytic and/or substrate binding properties | Mus musculus | ? | - |
? | |
additional information | the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell | Mus musculus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5,10-dideazatetrahydrofolic acid | L1210 isoform | Mus musculus | |
0.073 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
0.095 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
0.11 | - |
5-methyl-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
0.115 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus | |
0.116 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | L1210 isoform | Mus musculus | |
0.145 | - |
5,10-dideazatetrahydrofolic acid | liver isoform | Mus musculus | |
0.165 | - |
aminopterin | L1210 isoform | Mus musculus | |
0.212 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus | |
0.215 | - |
(6S)-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus | |
0.22 | - |
aminopterin | liver isoform | Mus musculus | |
0.22 | - |
5-methyl-5,6,7,8-tetrahydropteroylglutamate | liver isoform | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
0.0023 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
0.0024 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
0.0037 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
0.0042 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, L1210 isoform | Mus musculus | |
0.0052 | - |
(6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, liver isoform | Mus musculus | |
0.0083 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, L1210 isoform | Mus musculus | |
0.0083 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, L1210 isoform | Mus musculus | |
0.02 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
0.021 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
0.022 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
0.043 | - |
(6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, liver isoform | Mus musculus | |
0.054 | - |
(6S)-5,6,7,8-tetrahydropteroylpentaglutamate | Ki intercept, liver isoform | Mus musculus | |
0.055 | - |
(6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus | |
0.066 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, L1210 isoform | Mus musculus | |
0.084 | - |
(6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate | Ki slope, liver isoform | Mus musculus |