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Literature summary for 6.3.2.17 extracted from

  • Shane, B.
    Folylpoly-gamma-glutamate synthetase (1986), Chem. Biol. Pteridines, Proc. Int. Symp. Pteridines Folic Acid Deriv. Chem. Biol. Clin. Aspects, , 719-728.
No PubMed abstract available
Search for more literature for 6.3.2.17

Activating Compound

Activating Compound Comment Organism Structure
Reducing agents absolutely dependent on Sus scrofa
Reducing agents beta-mercaptoethanol or DTT Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 5,6,7,8-tetrahydropteroyl-Glu3 5,6,7,8-tetrahydropteroyl-Glu5 Sus scrofa
0.002
-
 5,6,7,8-tetrahydropteroyl-Glu4
-
 Sus scrofa
0.0022
-
 10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
0.0026
-
 7,8-dihydropteroyl-Glu2
-
 Sus scrofa
0.0027
-
 5,6,7,8-tetrahydropteroyl-Glu5
-
 Sus scrofa
0.0034
-
 5,6,7,8-tetrahydropteroyl-Glu2
-
 Sus scrofa
0.005
-
 7,8-dihydropteroyl-Glu
-
 Sus scrofa
0.0077
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
0.054
-
 methotrexate 5-methyl-5,6,7,8-tetrahydrpteroyl-Glu Sus scrofa
0.057
-
 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
 Sus scrofa
0.062
-
 pteroylmonoglutamyl-Glu
-
 Sus scrofa
0.093
-
 pteroylmonoglutamate
-
 Sus scrofa
0.119
-
 pteroyl-Glu3
-
 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required form maximal activity Escherichia coli
K+ required form maximal activity Sus scrofa
K+ required form maximal activity Corynebacterium sp.
K+ required form maximal activity Lacticaseibacillus casei
K+ absolutely dependent on monovalent cation, K+ is most effective Escherichia coli
K+ absolutely dependent on monovalent cation, K+ is most effective Sus scrofa
K+ absolutely dependent on monovalent cation, K+ is most effective Corynebacterium sp.
K+ absolutely dependent on monovalent cation, K+ is most effective Lacticaseibacillus casei
K+ maximal activity at 200 mM Escherichia coli
K+ maximal activity at 200 mM Sus scrofa
K+ maximal activity at 200 mM Corynebacterium sp.
K+ maximal activity at 200 mM Lacticaseibacillus casei
Mg2+ required Escherichia coli
Mg2+ required Sus scrofa
Mg2+ required Corynebacterium sp.
Mg2+ required Lacticaseibacillus casei
Mn2+
-
 Corynebacterium sp.
Mn2+ activates Sus scrofa
Mn2+ activates Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 gel filtration Lacticaseibacillus casei
46000
-
 gel filtration Escherichia coli
47000
-
 1 * 47000, SDS-PAGE Escherichia coli
51000
-
 gel filtration Corynebacterium sp.
53000
-
 1 * 53000, SDS-PAGE Corynebacterium sp.
62000
-
 1 * 62000, SDS-PAGE Sus scrofa
66000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Escherichia coli
-
-
-
Lacticaseibacillus casei
-
-
-
Sus scrofa
-
 hog
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Sus scrofa ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Escherichia coli ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Sus scrofa ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
 Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
-
 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
-
 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu5
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
-
 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 3 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 3 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 5 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 2 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 4 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Lacticaseibacillus casei ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 7,8-dihydropteroate + Glu
-
 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glu
-
 ?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
ATP + methotrexate + Glu
-
 Sus scrofa ADP + phosphate + methotrexyl-Glu
-
 ?
ATP + pteroyl-Glu + Glu n: 3 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
ATP + pteroyl-Glu + Glu n: 2 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
ATP + pteroyl-Glu + Glu n: 2 Lacticaseibacillus casei ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Lacticaseibacillus casei ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?
ATP + pteroyl-Glu3 + L-glutamate
-
 Sus scrofa ADP + phosphate + pteroyl-Glu4
-
 ?
additional information enzyme also posseses dihydrofolate synthetase activity Escherichia coli ?
-
 ?
additional information enzyme also posseses dihydrofolate synthetase activity Corynebacterium sp. ?
-
 ?
additional information no dihydrofolate synthetase activity Sus scrofa ?
-
 ?
additional information no dihydrofolate synthetase activity Lacticaseibacillus casei ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62000, SDS-PAGE Sus scrofa
monomer 1 * 53000, SDS-PAGE Corynebacterium sp.
monomer 1 * 43000, SDS-PAGE Lacticaseibacillus casei
monomer 1 * 47000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
additional information
-
 additional information
-
 Sus scrofa
additional information
-
 additional information
-
 Corynebacterium sp.
additional information
-
 additional information
-
 Lacticaseibacillus casei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
 Escherichia coli
9.4
-
-
 Sus scrofa
9.5
-
-
 Sus scrofa
9.5
-
-
 Corynebacterium sp.
9.5
-
-
 Lacticaseibacillus casei