Literature summary for 6.3.2.12 extracted from

Luraschi, A.; Cisse, O.H.; Monod, M.; Pagni, M.; Hauser, P.M.
Functional characterization of the Pneumocystis jirovecii potential drug targets dhfs and abz2 involved in folate biosynthesis (2015), Antimicrob. Agents Chemother., 59, 2560-2566 .

Application

Application	Comment	Organism
drug development	enzyme dihydrofolate synthase is a target for development of specific drugs against infections with the human pathogen Pneumocystis jirovecii that can cause pneumonia	Pneumocystis jirovecii

Cloned(Commentary)

Cloned (Comment)	Organism
gene dhfs, DNA and amino acid sequence determination and analysis, DNA is isolated from a bronchoalveolar lavage fluid sample of a patient, sequence comparisons, subcloning in Escherichia coli strain DH5alpha, heterologous expression of the enzyme can rescue of the null allele of the orthologous gene of Saccharomyces cerevisiae strain LCY1, functional complementation of the strain lacking the DHFS encoding gene FOL3	Pneumocystis jirovecii

Cloned (Comment)

Organism

gene dhfs, DNA and amino acid sequence determination and analysis, DNA is isolated from a bronchoalveolar lavage fluid sample of a patient, sequence comparisons, subcloning in Escherichia coli strain DH5alpha, heterologous expression of the enzyme can rescue of the null allele of the orthologous gene of Saccharomyces cerevisiae strain LCY1, functional complementation of the strain lacking the DHFS encoding gene FOL3

Pneumocystis jirovecii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Pneumocystis jirovecii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 7,8-dihydropteroate + L-glutamate	Pneumocystis jirovecii	i.e. 7,8-dihydropteroate	ADP + phosphate + 7,8-dihydropteroylglutamate	-	?
ATP + 7,8-dihydropteroate + L-glutamate	Pneumocystis jirovecii SE8	i.e. 7,8-dihydropteroate	ADP + phosphate + 7,8-dihydropteroylglutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pneumocystis jirovecii	L0PF87	-	-
Pneumocystis jirovecii SE8	L0PF87	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 7,8-dihydropteroate + L-glutamate	i.e. 7,8-dihydropteroate	Pneumocystis jirovecii	ADP + phosphate + 7,8-dihydropteroylglutamate	-	?
ATP + 7,8-dihydropteroate + L-glutamate	i.e. 7,8-dihydropteroate	Pneumocystis jirovecii SE8	ADP + phosphate + 7,8-dihydropteroylglutamate	-	?

Synonyms

Synonyms	Comment	Organism
DHFS	-	Pneumocystis jirovecii
PNEJI1_000945	-	Pneumocystis jirovecii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Pneumocystis jirovecii

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biosynthesis of folic acid and tetrahydrofolate, pathway overview. The DHFS enzyme carries out the final step of folic acid biosynthesis, namely, the addition of a glutamate to dihydropteroate to make folic acid (i.e. dihydrofolate)	Pneumocystis jirovecii
physiological function	the enzyme is essential for the life of the pathogen. The enzyme catalyzes the last step of folic acid, i.e. vitamin B9, biosynthesis	Pneumocystis jirovecii