Cloned (Comment) | Organism |
---|---|
gene folC, cloning and expression of wild-type and mutant enzymes with maltose binding protein-tag linked by a factor Xa cleavage site in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
A183P | naturally occuring conserved mutation, the mutant shows reduced enzyme activity | Mycobacterium tuberculosis variant bovis |
D112A | naturally occuring mutation | Mycobacterium tuberculosis |
E153A | naturally occuring conserved mutation, the mutant shows reduced enzyme activity | Mycobacterium tuberculosis |
E153G | naturally occuring conserved mutation | Mycobacterium tuberculosis |
E40G | naturally occuring mutation | Mycobacterium tuberculosis |
F152L | naturally occuring conserved mutation | Mycobacterium tuberculosis |
F152S | naturally occuring conserved mutation | Mycobacterium tuberculosis |
G111S | naturally occuring mutation | Mycobacterium tuberculosis |
I43A | naturally occuring mutation | Mycobacterium tuberculosis |
I43T | naturally occuring mutation, the mutant shows reduced enzyme activity | Mycobacterium tuberculosis |
additional information | 4-aminosalicylic acid susceptibility of mutant strains, overview | Mycobacterium tuberculosis |
N73S | naturally occuring conserved mutation | Mycobacterium tuberculosis |
R410W | naturally occuring mutation | Mycobacterium tuberculosis |
R49W | naturally occuring conserved mutation, the mutant shows reduced enzyme activity | Mycobacterium tuberculosis variant bovis |
S150G | naturally occuring conserved mutation | Mycobacterium tuberculosis |
S150R | naturally occuring conserved mutation | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 7,8-dihydropteroate + L-glutamate | Mycobacterium tuberculosis | - |
ADP + phosphate + 7,8-dihydropteroylglutamate | - |
? | |
ATP + 7,8-dihydropteroate + L-glutamate | Mycobacterium tuberculosis variant bovis | - |
ADP + phosphate + 7,8-dihydropteroylglutamate | - |
? | |
ATP + 7,8-dihydropteroate + L-glutamate | Mycobacterium tuberculosis H37Rv | - |
ADP + phosphate + 7,8-dihydropteroylglutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | I6Y0R5 | gene folC | - |
Mycobacterium tuberculosis H37Rv | I6Y0R5 | gene folC | - |
Mycobacterium tuberculosis variant bovis | A0A0H3M8P3 | gene folC | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes with maltose binding protein-tag linked by a factor Xa cleavage site from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, tag cleavage by faxtor Xa, dialysis, and anion exchange chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 7,8-dihydropteroate + L-glutamate | - |
Mycobacterium tuberculosis | ADP + phosphate + 7,8-dihydropteroylglutamate | - |
? | |
ATP + 7,8-dihydropteroate + L-glutamate | - |
Mycobacterium tuberculosis variant bovis | ADP + phosphate + 7,8-dihydropteroylglutamate | - |
? | |
ATP + 7,8-dihydropteroate + L-glutamate | - |
Mycobacterium tuberculosis H37Rv | ADP + phosphate + 7,8-dihydropteroylglutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FolC | - |
Mycobacterium tuberculosis |
FolC | - |
Mycobacterium tuberculosis variant bovis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis | |
ATP | - |
Mycobacterium tuberculosis variant bovis |
General Information | Comment | Organism |
---|---|---|
malfunction | in Mycobacterium bovis clinical isolates missense mutations within the coding sequence of the folC gene cause alterations within the 7,8-dihydropteroate binding pocket resulting in 4-aminosalicylic acid resistance. The alterations in the substrate binding pocket result in reduced dihydrofolate synthase activity and abolish the bioactivation of hydroxydihydropteroate to hydroxydihydrofolate. Introduction of a wild-type copy of folC fully restores PAS susceptibility in folC mutant strains | Mycobacterium tuberculosis variant bovis |
malfunction | in Mycobacterium tuberculosis clinical isolates missense mutations within the coding sequence of the folC gene cause alterations within the 7,8-dihydropteroate binding pocket resulting in 4-aminosalicylic acid resistance. The alterations in the substrate binding pocket result in reduced dihydrofolate synthase activity and abolish the bioactivation of hydroxydihydropteroate to hydroxydihydrofolate. Introduction of a wild-type copy of folC fully restores PAS susceptibility in folC mutant strains | Mycobacterium tuberculosis |