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Literature summary for 6.3.2.12 extracted from
- Purification and properties of the dihydrofolate synthetase from Serratia indica (1976), J. Nutr. Sci. Vitaminol., 22, 235-248.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Serratia indica |  |
| PCMB | - |
Serratia indica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required | Serratia indica | |
| Mg2+ | required | Serratia indica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Serratia indica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia indica | - |
IFO 3759 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia indica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0155 | - |
- |
Serratia indica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, unstable | Serratia indica |
| stable in the form of an ammonium sulfate precipitate in an ice bath for 3 months | Serratia indica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 7,8-dihydropteroate + L-Glu | - |
Serratia indica | ADP + phosphate + 7,8-dihydrofolate | - |
? | |
| GTP + 7,8-dihydropteroate + L-Glu | 37% of the activity relative to ATP | Serratia indica | GDP + phosphate + 7,8-dihydrofolate | - |
? | |
| ITP + 7,8-dihydropteroate + L-Glu | 59% of the activity relative to ATP | Serratia indica | IDP + phosphate + 7,8-dihydrofolate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, almost complete loss of activity | Serratia indica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Serratia indica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 11 | 7.5: about 30% of maximal activity, 11.0: about 70% of maximal activity | Serratia indica |
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Release 2023.1 (January 2023)
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