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Literature summary for 6.3.2.10 extracted from
- Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-g-glutamate ligases: identification of a ligase superfamily (1997), Biochemistry, 36, 6223-6229.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of naturally occuring mutant allele murF2 with A288T mutation as glutathione S-transferase fusion protein | Escherichia coli |
| expression of point mutants, expression of wild-type and naturally occuring mutant allele murF2 with A288T mutation as glutathione S-transferase fusion proteins | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A288T | naturally occuring murF2 allele, expressed as a fusion protein with glutathione S-transferase, is 181fold less catalytically active than the wild-type enzyme at 30°c and even more reduced at 42°C | Escherichia coli |
| E158A | site-directed mutagenesis, 4520fold reduced activity compared to the wild-type | Escherichia coli |
| E158D | site-directed mutagenesis, 246fold reduced activity compared to the wild-type | Escherichia coli |
| E158G | site-directed mutagenesis, over 451fold reduced activity compared to the wild-type | Escherichia coli |
| H188A | site-directed mutagenesis, 203fold reduced activity compared to the wild-type | Escherichia coli |
| H188D | site-directed mutagenesis, 2990fold reduced activity compared to the wild-type | Escherichia coli |
| H188G | site-directed mutagenesis, 214fold reduced activity compared to the wild-type | Escherichia coli |
| H188N | site-directed mutagenesis, 1860fold reduced activity compared to the wild-type | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | Escherichia coli | - |
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? |  |
| ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | Escherichia coli | part of bacterial peptidoglycan biosynthesis | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? | |
| additional information | Escherichia coli | the specific activity of the mutant enzyme is highly reduced compared to the wild-type murF | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P11880 | gene murF | - |
| Escherichia coli | P11880 | naturally occuring mutant murF2 allele, with A288T exchange | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | ordered kinetic mechanism, E158 and H188 are critical for enzyme activity | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04 | - |
purified recombinant enzyme, 42°C | Escherichia coli |
| 0.04 | - |
purified recombinant murF2 mutant, 42°C | Escherichia coli |
| 0.062 | - |
purified recombinant enzyme, 30°C | Escherichia coli |
| 0.062 | - |
purified recombinant murF2 mutant, 30°C | Escherichia coli |
| 11.2 | - |
purified recombinant wild-type enzyme, 30°C | Escherichia coli |
| 13.9 | - |
purified recombinant wild-type enzyme, 42°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | formation of an acyl phosphate intermediate | Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | part of bacterial peptidoglycan biosynthesis | Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? | |
| additional information | the specific activity of the mutant enzyme is highly reduced compared to the wild-type murF | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-Ala-D-Ala-adding enzyme | - |
Escherichia coli |
| More | member of ligase superfamily | Escherichia coli |
| Synthetase, uridine diphosphoacetylmuramoylpentapeptide | - |
Escherichia coli |
| UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase | - |
Escherichia coli |
| UDP-N-acetylmuramoylalanyl-D-glutamyl-lysine-D-alanyl-D-alanine ligase | - |
Escherichia coli |
| UDPacetylmuramoylpentapeptide synthetase | - |
Escherichia coli |
| Uridine diphosphoacetylmuramoylpentapeptide synthetase | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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