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Literature summary for 6.3.2.1 extracted from
- Pantothenate synthetase from Escherichia coli [D-pantoate:beta-alanine ligase (AMP-forming), EC 6.3.2.1] (1979), Methods Enzymol., 62, 215-219.
General Stability
| General Stability | Organism |
|---|---|
| lyophilized enzyme is very stable | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Escherichia coli |  |
| 4-Amino-3-hydroxybutanoate | 32% inhibition at 1 mM | Escherichia coli | |
| 4-aminobutanoate | 27% inhibition at 1 mM | Escherichia coli | |
| Ag+ | - |
Escherichia coli | |
| aspartate | 24% inhibition at 1 mM | Escherichia coli | |
| Ba2+ | - |
Escherichia coli | |
| Ca2+ | - |
Escherichia coli | |
| Co2+ | - |
Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| EDTA | - |
Escherichia coli | |
| EGTA | - |
Escherichia coli | |
| Fe2+ | slight | Escherichia coli | |
| gluconate | 32% inhibition at 1 mM | Escherichia coli | |
| glycolate | 30% inhibition at 1 mM | Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| methylmalonate | 38% inhibition at 1 mM | Escherichia coli | |
| Mg2+ | inhibition above 10 mM | Escherichia coli | |
| Mn2+ | inhibition above 5 mM | Escherichia coli | |
| Ni2+ | slight | Escherichia coli | |
| Pb2+ | slight | Escherichia coli | |
| Sn2+ | slight | Escherichia coli | |
| Sr2+ | slight | Escherichia coli | |
| taurine | 52% inhibition at 1 mM | Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
D-pantoate | - |
Escherichia coli | |
| 0.1 | - |
ATP | - |
Escherichia coli | |
| 0.15 | - |
beta-Alanine | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates | Escherichia coli | |
| K+ | Km: 5.5 mM | Escherichia coli | |
| Mg2+ | divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above | Escherichia coli | |
| Mn2+ | divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above | Escherichia coli | |
| NH4+ | activates | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
x * 18000, SDS-PAGE in presence of 2-mercaptoethanol | Escherichia coli |
| 69000 | - |
gel filtration | Escherichia coli |
| 70000 | - |
sedimentation equilibrium ultracentrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
IFO 13168 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.05 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 6 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-pantoate + beta-alanine | - |
Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 18000, SDS-PAGE in presence of 2-mercaptoethanol | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 5.0-10.0, 15 min stable | Escherichia coli |
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