Literature summary for 6.3.2.1 extracted from

Wang, S.; Eisenberg, D.
Crystal structures of a pantothenate synthetase from M. tuberculosis and its complexes with substrates and a reaction intermediate (2003), Protein Sci., 12, 1097-1108.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of native PS and PS complexed with alpha,beta-methyleneadenosine 5'-triphosphate, pantoate and the reaction intermediate pantoyl adenylate, crystals are grown by hanging-drop vapor diffusion, 0.003-0.005 ml of PS solution at 10 mg/ml PS is mixed with an equal volume of well solution, best crystals are obtained with well solutions containing 10%-15% polyethylene glycol 3000, 5% glycerol, 2% ethanol, 20 mM MgCl2, 150 mM Li2SO4 and 100 mM imidazole, pH 8.0 at 20°C, crystals diffract to 1.6-2.0 A	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

crystals of native PS and PS complexed with alpha,beta-methyleneadenosine 5'-triphosphate, pantoate and the reaction intermediate pantoyl adenylate, crystals are grown by hanging-drop vapor diffusion, 0.003-0.005 ml of PS solution at 10 mg/ml PS is mixed with an equal volume of well solution, best crystals are obtained with well solutions containing 10%-15% polyethylene glycol 3000, 5% glycerol, 2% ethanol, 20 mM MgCl2, 150 mM Li2SO4 and 100 mM imidazole, pH 8.0 at 20°C, crystals diffract to 1.6-2.0 A

Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
E77G	87% of wild-type activity	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31611	-	2 * 31611, mass spectroscopy, crystallization	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (R)-pantoate + beta-alanine	Mycobacterium tuberculosis	-	AMP + diphosphate + (R)-pantothenate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PS	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (R)-pantoate + beta-alanine	-	Mycobacterium tuberculosis	AMP + diphosphate + (R)-pantothenate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 31611, mass spectroscopy, crystallization	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
panthotenate synthetase	-	Mycobacterium tuberculosis
PS	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.74	-	(R)-pantoate	-	Mycobacterium tuberculosis
2.94	-	(R)-pantoate	-	Mycobacterium tuberculosis