KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
N8-glutathionylspermidine | - |
Crithidia fasciculata | |
0.02 | - |
N1-glutathionylspermidine | - |
Crithidia fasciculata | |
0.4 | - |
MgATP2- | - |
Crithidia fasciculata | |
0.914 | - |
gamma-L-Glu-L-Cys-Gly | - |
Crithidia fasciculata | |
1.07 | - |
spermidine | - |
Crithidia fasciculata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgATP2- required | Crithidia fasciculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
gel filtration | Crithidia fasciculata |
87000 | - |
1 * 87000, SDS-PAGE | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Crithidia fasciculata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP + H2O | - |
Crithidia fasciculata | N1,N8-bis-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate + NH3 | N1-glutathionylspermidine and N8-glutathionylspermidine are intermediates | ? | |
glutathione + spermidine + ATP | ATP in form of MgATP2- | Crithidia fasciculata | glutathionylspermidine + ADP + phosphate | - |
? | |
N8-glutathionylspermidine + glutathione + ATP | - |
Crithidia fasciculata | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 87000, SDS-PAGE | Crithidia fasciculata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
N1-glutathionylspermidine | - |
Crithidia fasciculata | |
24.3 | - |
N8-glutathionylspermidine | - |
Crithidia fasciculata | |
28.6 | - |
spermidine | - |
Crithidia fasciculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.8 | synthesis of both mono-glutathionylspermidine and di-glutathionylspermidine conjugates | Crithidia fasciculata |