Protein Variants | Comment | Organism |
---|---|---|
C57A | functional synthetase devoid of amidase activity. In the presence of inducer, this line showed decreased growth in vitro and decreased virulence in vivo, indicating that the amidase function is not absolutely required for viability | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
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Trypanosoma brucei | - |
- |
- |
General Information | Comment | Organism |
---|---|---|
physiological function | culture of a tetracyclinedependent conditional double knockout cDKO parasites without tetracycline induction results in loss of trypanothione and accumulation of glutathione, followed by growth inhibition and cell lysis after 6 days. In the absence of inducer, cDKO cells are unable to infect mice, confirming that the enzyme is essential for virulence in vivo as well as in vitro | Trypanosoma brucei |