Literature summary for 6.3.1.7 extracted from

  • Winter, H.C.; Ti-Zhi Su; Dekker, E.E.
    4-Methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts (1983), Biochem. Biophys. Res. Commun., 111, 484-489.
    View publication on PubMed

Inhibitors

Inhibitors Commentary Organism Structure
Asp with NH4+ is nitrogen donor Arachis hypogaea
Glu with NH4+ is nitrogen donor Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
8
-
4-methylene-L-glutamate about Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
280000
-
gel filtration Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility ID
ATP + 4-methylene-L-glutamate + NH3 Arachis hypogaea enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts ?
-
-
?

Organism

Organism UniProt Commentary Textmining
Arachis hypogaea
-
-
-

Purification (Commentary)

Purification (Commentary) Organism
partial Arachis hypogaea

Source Tissue

Source Tissue Commentary Organism Textmining
cotyledon
-
Arachis hypogaea
-

Substrates and Products (Substrate)

Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility Substrate Product ID
ATP + 4-methylene-L-glutamate + glutamine about 20% as effective as NH4+ 960 Arachis hypogaea AMP + diphosphate + glutamate + 4-methylene-L-glutamine
-
-
-
?
ATP + 4-methylene-L-glutamate + hydroxylamine 70% as effective as NH4+ 960 Arachis hypogaea AMP + diphosphate + 4-methylene-L-glutamylhydroxamate
-
-
-
?
ATP + 4-methylene-L-glutamate + NH3 enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts 960 Arachis hypogaea ?
-
-
-
?
ATP + 4-methylene-L-glutamate + NH4+
-
960 Arachis hypogaea AMP + diphosphate + 4-methylene-L-glutamine
-
960 Arachis hypogaea ?
additional information no amide/hydroxylamine exchange 960 Arachis hypogaea ?
-
-
-
?

pH Range

pH Minimum pH Maximum Commentary Organism
6.6 8.1 more than 70% of maximal activity at pH 6.6 and 8.1 Arachis hypogaea

Inhibitors (protein specific)

Inhibitors Commentary Organism Structure
Asp with NH4+ is nitrogen donor Arachis hypogaea
Glu with NH4+ is nitrogen donor Arachis hypogaea

KM Value [mM] (protein specific)

KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
8
-
4-methylene-L-glutamate about Arachis hypogaea

Molecular Weight [Da] (protein specific)

Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
280000
-
gel filtration Arachis hypogaea

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility ID
ATP + 4-methylene-L-glutamate + NH3 Arachis hypogaea enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts ?
-
-
?

Purification (Commentary) (protein specific)

Commentary Organism
partial Arachis hypogaea

Source Tissue (protein specific)

Source Tissue Commentary Organism Textmining
cotyledon
-
Arachis hypogaea
-

Substrates and Products (Substrate) (protein specific)

Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility ID
ATP + 4-methylene-L-glutamate + glutamine about 20% as effective as NH4+ 960 Arachis hypogaea AMP + diphosphate + glutamate + 4-methylene-L-glutamine
-
-
-
?
ATP + 4-methylene-L-glutamate + hydroxylamine 70% as effective as NH4+ 960 Arachis hypogaea AMP + diphosphate + 4-methylene-L-glutamylhydroxamate
-
-
-
?
ATP + 4-methylene-L-glutamate + NH3 enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts 960 Arachis hypogaea ?
-
-
-
?
ATP + 4-methylene-L-glutamate + NH4+
-
960 Arachis hypogaea AMP + diphosphate + 4-methylene-L-glutamine
-
960 Arachis hypogaea ?
additional information no amide/hydroxylamine exchange 960 Arachis hypogaea ?
-
-
-
?

pH Range (protein specific)

pH Minimum pH Maximum Commentary Organism
6.6 8.1 more than 70% of maximal activity at pH 6.6 and 8.1 Arachis hypogaea