Literature summary for 6.3.1.5 extracted from

Park, Y.; Yeo, H.; Lee, J.
Structural analysis of the NH3-dependent NAD+ synthetase from Deinococcus radiodurans (2014), Bull. Korean Chem. Soc., 35, 2863-2866 .

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
gene nadE, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) star pLysS	Deinococcus radiodurans

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant detagged enzyme, sitting drop vapor diffusion method, mixing of 30 mg/ml protein in 0.2 M NaCl, 20 mM Tris-HCl, pH 8.0, 1 mM DTT, 5 mM MgCl2, and 5% glycerol, with reservoir solution containing 20% PEG 4000, 0.2 M lithium sulfate, 0.1 M MES, pH 6.0, 23°C, 3 days, method optimization, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement	Deinococcus radiodurans

Crystallization (Comment)

Organism

purified recombinant detagged enzyme, sitting drop vapor diffusion method, mixing of 30 mg/ml protein in 0.2 M NaCl, 20 mM Tris-HCl, pH 8.0, 1 mM DTT, 5 mM MgCl2, and 5% glycerol, with reservoir solution containing 20% PEG 4000, 0.2 M lithium sulfate, 0.1 M MES, pH 6.0, 23°C, 3 days, method optimization, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement

Deinococcus radiodurans

Organism

Organism	UniProt	Comment	Textmining
Deinococcus radiodurans	Q9RYV5	-	-
Deinococcus radiodurans ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422	Q9RYV5	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) star pLysS by nickel affinity chromatography, tag cleavage by a protease, another step of nickel affinity chromatography, and gel filtration	Deinococcus radiodurans

Subunits

Subunits	Comment	Organism
More	enzyme structure analysis, overview	Deinococcus radiodurans

Synonyms

Synonyms	Comment	Organism
draNADS	-	Deinococcus radiodurans
NadE	-	Deinococcus radiodurans
NH3-dependent NAD+ synthetase	-	Deinococcus radiodurans

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Release 2023.1 (January 2023)