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Literature summary for 6.3.1.5 extracted from

  • Sorci, L.; Martynowski, D.; Rodionov, D.A.; Eyobo, Y.; Zogaj, X.; Klose, K.E.; Nikolaev, E.V.; Magni, G.; Zhang, H.; Osterman, A.L.
    Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis (2009), Proc. Natl. Acad. Sci. USA, 106, 3083-3088.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.9 A resolution Francisella tularensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 deamido-NMN
-
 Francisella tularensis
5.82
-
 deamido-NAD+
-
 Francisella tularensis

Organism

Organism UniProt Comment Textmining
Francisella tularensis A0A0B3VMT3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + NH3
-
 Francisella tularensis AMP + diphosphate + NAD+
-
 ?
ATP + deamido-NMN + NH3 existence of an alternative route of NAD synthesis where the amidation of NaMN to nicotinamide mononucleotide occurs before the adenylylation reaction, which converts the intermediate to the NAD cofactor. The first step is catalyzed by Francisella tularensis NadE Francisella tularensis AMP + diphosphate + NMN
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 deamido-NAD+
-
 Francisella tularensis
0.5
-
 deamido-NMN
-
 Francisella tularensis

General Information

General Information Comment Organism
physiological function existence of an alternative route of NAD synthesis where the amidation of NaMN to nicotinamide mononucleotide occurs before the adenylylation reaction, which converts the intermediate to the NAD cofactor. The first step is catalyzed by Francisella tularensis NadE Francisella tularensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
43
-
 deamido-NAD+
-
 Francisella tularensis
2500
-
 deamido-NMN
-
 Francisella tularensis