Cloned (Comment) | Organism |
---|---|
overexpression in strain BL21(DE3) as HIs-tagged enzyme | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
28 mg/ml purified recombinant enzyme, pure or in complex with substrates NAD+ and ATP, hanging-drop vapour diffusion method, 22°C room temperature, protein in 0.01 M HEPES, pH 7.5, 5 mM MgCl2, 3 mM DTT, precipitant solution: 0.1 M HEPES, pH 7.5, 1.5 M lithium sulfate, X-ray diffraction structure determination at 2.0 A resolution, compex structure determination via molecular replacement method | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | slightly inhibiting above 5 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required at 1.5 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + glutamine | Escherichia coli | - |
AMP + diphosphate + glutamate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | Escherichia coli | - |
AMP + diphosphate + NAD+ | - |
? | |
additional information | Escherichia coli | 2 possible substrates, NH3 or glutamine, to perform the final step in the Preiss-Handler pathway for NAD+ biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinantly overexpressed enzyme, to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + glutamine | - |
Escherichia coli | AMP + diphosphate + glutamate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | - |
Escherichia coli | AMP + diphosphate + NAD+ | - |
? | |
additional information | 2 possible substrates, NH3 or glutamine, to perform the final step in the Preiss-Handler pathway for NAD+ biosynthesis | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
NAD synthase | - |
Escherichia coli |
NAD synthetase | - |
Escherichia coli |
Nicotinamide adenine dinucleotide synthetase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
active in a broad pH range | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |