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Literature summary for 6.3.1.5 extracted from

  • Ozment, C.; Barchue, J.; DeLucas, L.J.; Chattopadhyay, D.
    Structural study of Escherichia coli NAD synthetase: overexpression, purification, crystallization, and preliminary crystallographic analysis (1999), J. Struct. Biol., 127, 279-282.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in strain BL21(DE3) as HIs-tagged enzyme Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
28 mg/ml purified recombinant enzyme, pure or in complex with substrates NAD+ and ATP, hanging-drop vapour diffusion method, 22°C room temperature, protein in 0.01 M HEPES, pH 7.5, 5 mM MgCl2, 3 mM DTT, precipitant solution: 0.1 M HEPES, pH 7.5, 1.5 M lithium sulfate, X-ray diffraction structure determination at 2.0 A resolution, compex structure determination via molecular replacement method Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ slightly inhibiting above 5 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required at 1.5 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, recombinant enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deamido-NAD+ + glutamine Escherichia coli
-
AMP + diphosphate + glutamate + NAD+
-
?
ATP + deamido-NAD+ + NH3 Escherichia coli
-
AMP + diphosphate + NAD+
-
?
additional information Escherichia coli 2 possible substrates, NH3 or glutamine, to perform the final step in the Preiss-Handler pathway for NAD+ biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinantly overexpressed enzyme, to homogeneity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + glutamine
-
Escherichia coli AMP + diphosphate + glutamate + NAD+
-
?
ATP + deamido-NAD+ + NH3
-
Escherichia coli AMP + diphosphate + NAD+
-
?
additional information 2 possible substrates, NH3 or glutamine, to perform the final step in the Preiss-Handler pathway for NAD+ biosynthesis Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 35000, recombinant enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
NAD synthase
-
Escherichia coli
NAD synthetase
-
Escherichia coli
Nicotinamide adenine dinucleotide synthetase
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
active in a broad pH range Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli