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Literature summary for 6.3.1.5 extracted from

  • Veiga-Malta, I.; Duarte, M.; Dinis, M.; Madureira, P.; Ferreira, P.; Videira, A.
    Identification of NAD+ synthetase from Streptococcus sobrinus as a B-cell-stimulatory protein (2004), J. Bacteriol., 186, 419-426.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
functional expression in Escherichia coli strain M-15 as His-tagged enzyme, secretion to the medium Streptococcus sobrinus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion Streptococcus sobrinus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus sobrinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, recombinant His-tagged enzyme, SDS-PAGE Streptococcus sobrinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deamido-NAD+ + NH3 Streptococcus sobrinus
-
AMP + diphosphate + NAD+
-
?
ATP + deamido-NAD+ + NH3 Streptococcus sobrinus 6715
-
AMP + diphosphate + NAD+
-
?
additional information Streptococcus sobrinus enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures ?
-
?
additional information Streptococcus sobrinus 6715 enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures ?
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus sobrinus
-
-
-
Streptococcus sobrinus 6715
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant as His-tagged enzyme from Escherichia coli Streptococcus sobrinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.49
-
purified recombinant His-tagged enzyme Streptococcus sobrinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + NH3
-
Streptococcus sobrinus AMP + diphosphate + NAD+
-
?
ATP + deamido-NAD+ + NH3
-
Streptococcus sobrinus 6715 AMP + diphosphate + NAD+
-
?
additional information enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures Streptococcus sobrinus ?
-
?
additional information enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures Streptococcus sobrinus 6715 ?
-
?

Subunits

Subunits Comment Organism
? x * 38000, recombinant His-tagged enzyme, SDS-PAGE Streptococcus sobrinus

Synonyms

Synonyms Comment Organism
NAD synthase
-
Streptococcus sobrinus
NAD synthetase
-
Streptococcus sobrinus
Nicotinamide adenine dinucleotide synthetase
-
Streptococcus sobrinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Streptococcus sobrinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
10 min, inactivation Streptococcus sobrinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Streptococcus sobrinus

Cofactor

Cofactor Comment Organism Structure
ATP required Streptococcus sobrinus