Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
NH3 | pH 8.5, 37°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal at 3 mM | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, SDS-PAGE | Geobacillus stearothermophilus |
50000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | Geobacillus stearothermophilus | - |
AMP + diphosphate + NAD+ | - |
ir | |
ATP + deamido-NAD+ + NH3 | Geobacillus stearothermophilus H-804 | - |
AMP + diphosphate + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | E07950 | H-804 | - |
Geobacillus stearothermophilus H-804 | E07950 | H-804 | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.59 | - |
purified native enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | - |
Geobacillus stearothermophilus | AMP + diphosphate + NAD+ | - |
ir | |
ATP + deamido-NAD+ + NH3 | specific for NH3, no activity with L-glutamine or other L-amino acids, no activity with urea, uric acid, or creatinine | Geobacillus stearothermophilus | AMP + diphosphate + NAD+ | - |
ir | |
ATP + deamido-NAD+ + NH3 | - |
Geobacillus stearothermophilus H-804 | AMP + diphosphate + NAD+ | - |
ir | |
ATP + deamido-NAD+ + NH3 | specific for NH3, no activity with L-glutamine or other L-amino acids, no activity with urea, uric acid, or creatinine | Geobacillus stearothermophilus H-804 | AMP + diphosphate + NAD+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
ammonia-specific NAD synthetase | - |
Geobacillus stearothermophilus |
NAD synthase | - |
Geobacillus stearothermophilus |
NAD synthetase | - |
Geobacillus stearothermophilus |
Nicotinamide adenine dinucleotide synthetase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable at | Geobacillus stearothermophilus |
70 | - |
40% of the activity at 60°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | isoelectric focusing | - |
5.3 |