Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.1.2 extracted from

  • Kumar, V.; Yadav, S.; Soumya, N.; Kumar, R.; Babu, N.K.; Singh, S.
    Biochemical and inhibition studies of glutamine synthetase from Leishmania donovani (2017), Microb. Pathog., 107, 164-174 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene LdGS, the enzyme exists as a single copy gene in parasite genome, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
L-methionine sulfone
-
Leishmania donovani
L-methionine sulfoximine
-
Leishmania donovani
Phosphinothricin
-
Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
26.3
-
L-glutamate recombinant enzyme, pH 7.8, 37°C Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Leishmania donovani 5737
-
additional information presence of mitochondrial targeting sequence and the cleavage localization signal at the N-terminal region in LdGS protein Leishmania donovani
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially substitute for Mg2+ Leishmania donovani
Mg2+ required Leishmania donovani
Mn2+ can partially substitute for Mg2+ Leishmania donovani
Ni2+ can partially substitute for Mg2+ Leishmania donovani
Zn2+ can partially substitute for Mg2+ Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + NH3 Leishmania donovani
-
ADP + phosphate + L-glutamine
-
r
ATP + L-glutamate + NH3 Leishmania donovani BPK282A1
-
ADP + phosphate + L-glutamine
-
r

Organism

Organism UniProt Comment Textmining
Leishmania donovani E9B8P8 MHOM/80/IN/Dd8
-
Leishmania donovani BPK282A1 E9B8P8 MHOM/80/IN/Dd8
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
Leishmania donovani
-
promastigote
-
Leishmania donovani
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.322
-
enzyme from promastigotes, pH 7.8, 37°C Leishmania donovani
0.7325
-
recombinant His-tagged enzyme, pH 7.8, 37°C Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH3
-
Leishmania donovani ADP + phosphate + L-glutamine
-
r
ATP + L-glutamate + NH3
-
Leishmania donovani BPK282A1 ADP + phosphate + L-glutamine
-
r

Subunits

Subunits Comment Organism
? x * 43500, recombinant His-tagged enzyme, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
LDBPK_060370
-
Leishmania donovani
LdGS
-
Leishmania donovani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Leishmania donovani

pH Range

pH Minimum pH Maximum Comment Organism
6 11 activity range Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
ATP
-
Leishmania donovani