Cloned (Comment) | Organism |
---|---|
gene LdGS, the enzyme exists as a single copy gene in parasite genome, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine sulfone | - |
Leishmania donovani | |
L-methionine sulfoximine | - |
Leishmania donovani | |
Phosphinothricin | - |
Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.3 | - |
L-glutamate | recombinant enzyme, pH 7.8, 37°C | Leishmania donovani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Leishmania donovani | 5737 | - |
additional information | presence of mitochondrial targeting sequence and the cleavage localization signal at the N-terminal region in LdGS protein | Leishmania donovani | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially substitute for Mg2+ | Leishmania donovani | |
Mg2+ | required | Leishmania donovani | |
Mn2+ | can partially substitute for Mg2+ | Leishmania donovani | |
Ni2+ | can partially substitute for Mg2+ | Leishmania donovani | |
Zn2+ | can partially substitute for Mg2+ | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | Leishmania donovani | - |
ADP + phosphate + L-glutamine | - |
r | |
ATP + L-glutamate + NH3 | Leishmania donovani BPK282A1 | - |
ADP + phosphate + L-glutamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | E9B8P8 | MHOM/80/IN/Dd8 | - |
Leishmania donovani BPK282A1 | E9B8P8 | MHOM/80/IN/Dd8 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Leishmania donovani | - |
promastigote | - |
Leishmania donovani | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.322 | - |
enzyme from promastigotes, pH 7.8, 37°C | Leishmania donovani |
0.7325 | - |
recombinant His-tagged enzyme, pH 7.8, 37°C | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | - |
Leishmania donovani | ADP + phosphate + L-glutamine | - |
r | |
ATP + L-glutamate + NH3 | - |
Leishmania donovani BPK282A1 | ADP + phosphate + L-glutamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 43500, recombinant His-tagged enzyme, SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
LDBPK_060370 | - |
Leishmania donovani |
LdGS | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Leishmania donovani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | activity range | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Leishmania donovani |