Cloned (Comment) | Organism |
---|---|
from 21-day schistosomes, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, quantitative real-time PCR expression analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Schistosoma japonicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Schistosoma japonicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40700 | - |
x * 45000, recombinant enzyme, SDS-PAGE, x * 40700, about, sequence calculation | Schistosoma japonicum |
45000 | - |
x * 45000, recombinant enzyme, SDS-PAGE, x * 40700, about, sequence calculation | Schistosoma japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | Schistosoma japonicum | - |
ADP + phosphate + L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma japonicum | Q86EI1 | Chinese mainland strain, Anhui isolate | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Schistosoma japonicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
integument | in 28-day adult worms | Schistosoma japonicum | - |
additional information | schistosomes are maintained in New Zealand rabbits. Quantitative real-time PCR expression analysis of enzyme in worms of different stages, overview | Schistosoma japonicum | - |
parenchyma | in 28-day adult worms | Schistosoma japonicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3300 | - |
purified recombinant enzyme, pH 7.9, 37°C | Schistosoma japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | - |
Schistosoma japonicum | ADP + phosphate + L-glutamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, recombinant enzyme, SDS-PAGE, x * 40700, about, sequence calculation | Schistosoma japonicum |
Synonyms | Comment | Organism |
---|---|---|
Glutamine synthetase | - |
Schistosoma japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Schistosoma japonicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | purified recombinant enzyme, stable | Schistosoma japonicum |
50 | - |
purified recombinant enzyme, enzyme activity decreases rapidly at 50°C | Schistosoma japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Schistosoma japonicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | purified recombinant enzyme, stable | Schistosoma japonicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Schistosoma japonicum |
Organism | Comment | Expression |
---|---|---|
Schistosoma japonicum | transcription of SjGS is upregulated in praziquantel-treated worms at 2-, 4-, and 24-h posttreatment | up |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme sequence contains a classic beta-grasp domain and a catalytic domain of glutamine synthetase | Schistosoma japonicum |
physiological function | glutamine synthetase catalyzes the synthesis of glutamine, providing nitrogen for the production of purines, pyrimidines, amino acids, and other compounds required in many pivotal cellular events. The enzyme is important in the development of the schistosome | Schistosoma japonicum |