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Literature summary for 6.3.1.2 extracted from

  • Ehlers, C.; Weidenbach, K.; Veit, K.; Forchhammer, K.; Schmitz, R.A.
    Unique mechanistic features of post-translational regulation of glutamine synthetase activity in Methanosarcina mazei strain Gö1 in response to nitrogen availability (2005), Mol. Microbiol., 55, 1841-1854.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
nitrogen sensor protein GlnK1 the nitrogen sensor protein GlnK1 of the methanogenic archaeon interacts and forms stable complexes with glutamine synthetase GlnA1. Complex formation with GlnK1 in the absence of metabolites inhibits the activity of GlnA1. The nitrogen sensor GlnK1 allows finetuning control of the glutamine synthetase activity under changing nitrogen availabilities. The following model is proposed: under nitrogen limitation, increasing concentrations of 2-oxoglutarate stimulate maximal GlnA1 activity and transform GlnA1 into an activated conformation, which prevents inhibition by GlnK1. Upon a shift to nitrogen sufficiency after a period of nitrogen limitation, GlnA1 activity is reduced by decreasing internal 2-oxoglutarate concentrations through diminished direct activation and by GlnK1 inhibition Methanosarcina mazei

Metals/Ions

Metals/Ions Comment Organism Structure
2-oxoglutarate directly stimulates activity Methanosarcina mazei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, calculated from sequence Methanosarcina mazei

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei Q8PY99
-
-
Methanosarcina mazei DSM 3647 Q8PY99
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH3
-
Methanosarcina mazei ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3
-
Methanosarcina mazei DSM 3647 ADP + phosphate + L-glutamine
-
?

Subunits

Subunits Comment Organism
? x * 45000, calculated from sequence Methanosarcina mazei

Synonyms

Synonyms Comment Organism
GlnA1
-
Methanosarcina mazei
MM_0964 locus name Methanosarcina mazei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methanosarcina mazei