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Literature summary for 6.3.1.2 extracted from

  • Chandra, H.; Basir, S.F.; Gupta, M.; Banerjee, N.
    Glutamine synthetase encoded by glnA-1 is necessary for cell wall resistance and pathogenicity of Mycobacterium bovis (2010), Microbiology, 156, 3669-3677.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis variant bovis
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Source Tissue

Source Tissue Comment Organism Textmining

Synonyms

Synonyms Comment Organism
glnA-1
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Mycobacterium tuberculosis variant bovis

General Information

General Information Comment Organism
physiological function study on glnA-1 mutant that produces reduced levels of glutamine synthetase. The mutant is able to grow in enriched 7H9 medium without glutamine supplementation.The glnA-1 strain contains no detectable poly-alpha-L-glutamine in the cell walls and shows marked sensitivity to different chemical and physical stresses such as lysozyme, SDS and sonication. The sensitivity of the mutant to antitubercular drugs, rifampicin and D-cycloserine, is also increased. The glnA-1 strain infects THP-1 cells with reduced efficiency and is also attenuated for growth in macrophages. A Mycobacterium smegmatis strain containing the Mycobacterium bovis glnA-1 gene survives longer in THP-1 cells than the wild-type strain and also produces cell wall-associated poly-alpha-L-glutamine. The mutant is not able to replicate in the organs of BALB/c mice and is cleared within 4-6 weeks of infection. Disruption of the glnA-1 gene adversely affects biofilm formation on polystyrene surfaces Mycobacterium tuberculosis variant bovis