Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Zea mays |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, GS crystals in complex with ADP and methionine sulfoximine phosphate, using 9% (w/v) polyethylene glycol 8000, 100 mM Tris-HCl pH 7.8, 5% (v/v) 2-methyl-2,4-pentanediol, 10 mM MnCl2 | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | essential for activity, contains three Mn2+ ions per subunit | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Zea mays | ADP + phosphate + L-Gln | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | x-ray crystallography | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
Glutamine synthetase | - |
Zea mays |
GS1a | - |
Zea mays |