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Literature summary for 6.3.1.2 extracted from

  • Unno, H.; Uchida, T.; Sugawara, H.; Kurisu, G.; Sugiyama, T.; Yamaya, T.; Sakakibara, H.; Hase, T.; Kusunoki, M.
    Atomic structure of plant glutamine synthetase: a key enzyme for plant productivity (2006), J. Biol. Chem., 281, 29287-29296.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Zea mays

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, GS crystals in complex with ADP and methionine sulfoximine phosphate, using 9% (w/v) polyethylene glycol 8000, 100 mM Tris-HCl pH 7.8, 5% (v/v) 2-methyl-2,4-pentanediol, 10 mM MnCl2 Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ essential for activity, contains three Mn2+ ions per subunit Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
Zea mays ADP + phosphate + L-Gln
-
?

Subunits

Subunits Comment Organism
decamer x-ray crystallography Zea mays

Synonyms

Synonyms Comment Organism
Glutamine synthetase
-
Zea mays
GS1a
-
Zea mays