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Literature summary for 6.3.1.2 extracted from

  • Ishiyama, K.; Inoue, E.; Tabuchi, M.; Yamaya, T.; Takahashi, H.
    Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots (2004), Plant Cell Physiol., 45, 1640-1647.
    View publication on PubMed
Search for more literature for 6.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
expression in Escherichia coli, isoenzyme OsGLN1,2 Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 NH4+ 30°C, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
0.073
-
 NH4+ 30°C, isoenzyme OsGLN1,2 Oryza sativa Japonica Group
0.45
-
 ATP 30°C, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
0.53
-
 ATP 30°C, isoenzyme OsGLN1,2 Oryza sativa Japonica Group
1.9
-
 L-glutamate 30°C, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
2.1
-
 L-Glu 30°C, isoenzyme OsGLN1,2 Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Glu + NH4+ Oryza sativa Japonica Group serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess ADP + phosphate + L-Gln
-
 ?
ATP + L-glutamate + NH4+ Oryza sativa Japonica Group serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess ADP + phosphate + L-glutamine
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group P14654 isoenzyme OsGLN1,2
-
Oryza sativa Japonica Group P14656 isoenzyme OsGLN1,1
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme OsGLN1,1 Oryza sativa Japonica Group
isoenzyme OsGLN1,2 Oryza sativa Japonica Group

Source Tissue

Source Tissue Comment Organism Textmining
epidermis OsGLN1,1, under nitrogen-limited condition Oryza sativa Japonica Group
-
epidermis OsGLN1,2, under nitrogen-sufficient condition Oryza sativa Japonica Group
-
exodermis OsGLN1,1, under nitrogen-limited condition Oryza sativa Japonica Group
-
exodermis OsGLN1,2, under nitrogen-sufficient condition Oryza sativa Japonica Group
-
root OsGLN1,1 is expressed in epidermis and exodermis under nitrogen-limited condition. Within the central cylinder of the elongating zone the enzyme is induced by ammonium Oryza sativa Japonica Group
-
root OsGLN1,2 is expressed in epidermis and exodermis under nitrogen-sufficient condition. Within the central cylinder of the elongating zone the enzyme is induced by ammonium Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Oryza sativa Japonica Group ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+ serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess Oryza sativa Japonica Group ADP + phosphate + L-Gln
-
 ?
ATP + L-glutamate + NH4+
-
 Oryza sativa Japonica Group ADP + phosphate + L-glutamine
-
 ?
ATP + L-glutamate + NH4+ serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess Oryza sativa Japonica Group ADP + phosphate + L-glutamine
-
 ?

Synonyms

Synonyms Comment Organism
OsGLN1,1
-
 Oryza sativa Japonica Group
OsGLN1,2
-
 Oryza sativa Japonica Group