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Literature summary for 6.3.1.2 extracted from

  • Ishiyama, K.; Inoue, E.; Watanabe-Takahashi, A.; Obara, M.; Yamaya, T.; Takahashi, H.
    Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis (2004), J. Biol. Chem., 279, 16598-16605.
    View publication on PubMed
Search for more literature for 6.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoenzyme GLN1,1 in Escherichia coli Arabidopsis thaliana
expression of isoenzyme GLN1,2 in Escherichia coli Arabidopsis thaliana
expression of isoenzyme GLN1,3 in Escherichia coli Arabidopsis thaliana
expression of isoenzyme GLN1,4 in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
glutamate at high concentrations, isoenzyme GLN1,3 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NH4+ pH 7.8, 30°C, below, isoenzyme GLN1,1 Arabidopsis thaliana
0.3
-
 ATP pH 7.8, 30°C, isoenzyme GLN1,1 Arabidopsis thaliana
0.56
-
 ATP pH 7.8, 30°C, isoenzyme GLN1,4 Arabidopsis thaliana
0.58
-
 NH4+ pH 7.8, 30°C, below, isoenzyme GLN1,4 Arabidopsis thaliana
0.6
-
 L-glutamate pH 7.8, 30°C, isoenzyme GLN1,4 Arabidopsis thaliana
0.85
-
 ATP pH 7.8, 30°C, isoenzyme GLN1,3 Arabidopsis thaliana
1.1
-
 L-glutamate pH 7.8, 30°C, isoenzyme GLN1,1 Arabidopsis thaliana
1.1
-
 ATP pH 7.8, 30°C, isoenzyme GLN1,2 Arabidopsis thaliana
1.21
-
 NH4+ pH 7.8, 30°C, below, isoenzyme GLN1,3 Arabidopsis thaliana
2.45
-
 NH4+ pH 7.8, 30°C, below, isoenzyme GLN1,2 Arabidopsis thaliana
3.8
-
 L-glutamate pH 7.8, 30°C, isoenzyme GLN1,2 Arabidopsis thaliana
3.9
-
 L-glutamate pH 7.8, 30°C, isoenzyme GLN1,3 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + NH4+ Arabidopsis thaliana glutamine synthetase is a key enzyme in nitrogen assimilation ADP + phosphate + L-glutamine
-
 ?
ATP + L-glutamate + NH4+ Arabidopsis thaliana glutamine synthetase is a key enzyme in nitrogen assimilation. Isoenzyme GLN1,1 accumulates in the surface layers of root during nitrogen limitation ADP + phosphate + L-glutamine
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 isoenzyme GLN1,2
-
Arabidopsis thaliana Q56WN1 isoenzyme GLN1,1
-
Arabidopsis thaliana Q9FMD9 isoenzyme GLN1,4
-
Arabidopsis thaliana Q9LVI8 isoenzyme GLN1,3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isoenzyme GLN1,1 Arabidopsis thaliana
recombinant isoenzyme GLN1,2 Arabidopsis thaliana
recombinant isoenzyme GLN1,3 Arabidopsis thaliana
recombinant isoenzyme GLN1,4 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf isoenzyme GLN1,1 Arabidopsis thaliana
-
leaf isoenzyme GLN1,2 Arabidopsis thaliana
-
leaf isoenzyme GLN1,3 Arabidopsis thaliana
-
additional information expressed in nitrogen-starved plants, but 10fold less abundant than isoenzyme GLN1,1 Arabidopsis thaliana
-
root isoenzyme GLN1,1 accumulates in the surface layers of root during nitrogen limitation Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH4+ glutamine synthetase is a key enzyme in nitrogen assimilation Arabidopsis thaliana ADP + phosphate + L-glutamine
-
 ?
ATP + L-glutamate + NH4+ glutamine synthetase is a key enzyme in nitrogen assimilation. Isoenzyme GLN1,1 accumulates in the surface layers of root during nitrogen limitation Arabidopsis thaliana ADP + phosphate + L-glutamine
-
 ?

Synonyms

Synonyms Comment Organism
GLN1,1
-
 Arabidopsis thaliana
GLN1,2
-
 Arabidopsis thaliana
GLN1,3
-
 Arabidopsis thaliana
GLN1,4
-
 Arabidopsis thaliana