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Literature summary for 6.3.1.2 extracted from

  • Yamamoto, S.; Uchimura, K.; Wakayama, M.; Tachiki, T.
    Purification and characterization of glutamine synthetase of Pseudomonas taetrolens Y-30: an enzyme usable for production of theanine by coupling with the alcoholic fermentation system of baker's yeast (2004), Biosci. Biotechnol. Biochem., 68, 1888-1897.
    View publication on PubMed
Search for more literature for 6.3.1.2

Application

Application Comment Organism
synthesis isolated glutamine synthetase forms theanine in the mixture with the yeast fermentation system, production in high concentrations can be achieved Pseudomonas taetrolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas taetrolens
0.56
-
 NH4+ pH 8.0, 30 mM Mg2+ Pseudomonas taetrolens
0.59
-
 NH4+ pH 7.5, 3 mM MN2+ Pseudomonas taetrolens
1.3
-
 ATP pH 11.0, 30 mM Mg2+ Pseudomonas taetrolens
1.4
-
 ATP pH 8.0, 3 mM Mn2+ Pseudomonas taetrolens
1.4
-
 ATP pH 8.5, 30 mM Mg2+ Pseudomonas taetrolens
1.5
-
 ATP pH 7.5, 3 mM MN2+ Pseudomonas taetrolens
1.5
-
 ATP pH 8.5, 3 mM Mn2+ Pseudomonas taetrolens
2
-
 ATP pH 8.0, 30 mM Mg2+ Pseudomonas taetrolens
20
-
 methylamine pH 8.0, 3 mM Mn2+ Pseudomonas taetrolens
100
-
 methylamine pH 8.5, 30 mM Mg2+ Pseudomonas taetrolens
500
-
 ethylamine pH 8.5, 3 mM Mn2+ Pseudomonas taetrolens
700
-
 ethylamine pH 11.0, 30 mM Mg2+ Pseudomonas taetrolens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, optimum: 15 mM Pseudomonas taetrolens
Mn2+ activates with 11% of the efficiency of the activation with Mg2+, optimum: 3-4 mM Pseudomonas taetrolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 12 * 55000, SDS-PAGE Pseudomonas taetrolens
66000
-
 gel filtration Pseudomonas taetrolens

Organism

Organism UniProt Comment Textmining
Pseudomonas taetrolens
-
 srain Y-30
-
Pseudomonas taetrolens Y-30
-
 srain Y-30
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas taetrolens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17
-
-
 Pseudomonas taetrolens

Storage Stability

Storage Stability Organism
-20°C, stable for two months Pseudomonas taetrolens
5°C, about 95% of the initial activity is maintained for 30 days in a state of ammonium sulfate precipitate (70% saturation) Pseudomonas taetrolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + ethylamine 1% of the activity with NH4+, at pH 8.0, activated with 30 mM Mg2+ Pseudomonas taetrolens ADP + phosphate + L-Gln + ?
-
 ?
ATP + L-Glu + ethylamine 1% of the activity with NH4+, at pH 8.0, activated with 30 mM Mg2+ Pseudomonas taetrolens Y-30 ADP + phosphate + L-Gln + ?
-
 ?
ATP + L-Glu + hydroxylamine 32% of the activity with NH4+ Pseudomonas taetrolens ADP + phosphate + L-Gln + ?
-
 ?
ATP + L-Glu + hydroxylamine 32% of the activity with NH4+ Pseudomonas taetrolens Y-30 ADP + phosphate + L-Gln + ?
-
 ?
ATP + L-Glu + methylamine 7% of the activity with NH4+, at pH 8.0, activated with 30 mM Mg2+ Pseudomonas taetrolens ADP + phosphate + L-Gln + ?
-
 ?
ATP + L-Glu + NH4+
-
 Pseudomonas taetrolens ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+
-
 Pseudomonas taetrolens Y-30 ADP + phosphate + L-Gln
-
 ?
GTP + L-Glu + NH4+ 26% of the activity with ATP Pseudomonas taetrolens GDP + phosphate + L-Gln
-
 ?
GTP + L-Glu + NH4+ 26% of the activity with ATP Pseudomonas taetrolens Y-30 GDP + phosphate + L-Gln
-
 ?
ITP + L-Glu + NH4+ 18% of the activity with ATP Pseudomonas taetrolens IDP + phosphate + L-Gln
-
 ?
ITP + L-Glu + NH4+ 18% of the activity with ATP Pseudomonas taetrolens Y-30 IDP + phosphate + L-Gln
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 55000, SDS-PAGE Pseudomonas taetrolens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable Pseudomonas taetrolens
65
-
 10 min, 40% loss of activity Pseudomonas taetrolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 activated with 3 mM Mn2+ Pseudomonas taetrolens
8
-
 activated by 30 mM Mg2+ Pseudomonas taetrolens
8.5
-
 Mn2+-dependent reaction with ethylamine Pseudomonas taetrolens
11
-
 Mg2+-dependent reaction with ethylamine Pseudomonas taetrolens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 stable Pseudomonas taetrolens