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Literature summary for 6.3.1.2 extracted from

  • Montanini, B.; Betti, M.; Marquez, A.J.; Balestrini, R.; Bonfante, P.; Ottonello, S.
    Distinctive properties and expression profiles of glutamine synthetase from a plant symbiotic fungus (2003), Biochem. J., 373, 357-368.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Tuber borchii

Inhibitors

Inhibitors Comment Organism Structure
L-glutamine
-
 Tuber borchii
L-methionine sulfoximine 50% inhibition at 47 mM Tuber borchii
L-phosphinothricin 50% inhibition at 0.5 mM Tuber borchii
Mg2+
-
 Tuber borchii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 ATP 37°C, pH 7.5 Tuber borchii
0.75
-
 NH4+ 37°C, pH 7.5 Tuber borchii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39700
-
 calculated from amino acid sequence Tuber borchii
42000
-
 purified recombinant protein containing a hexahistidine-tag Tuber borchii
340000
-
 gel filtration, minor peak at 175000 Da Tuber borchii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glutamate + NH4+ Tuber borchii central enzyme of nitrogen metabolism, involved in nitrogen assimilation pathway ADP + phosphate + L-glutamine
-
 ?

Organism

Organism UniProt Comment Textmining
Tuber borchii Q86ZU6 strain ATCC 95640
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Tuber borchii

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Tuber borchii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glutamate + NH4+
-
 Tuber borchii ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, involved in nitrogen assimilation pathway Tuber borchii ADP + phosphate + L-glutamine
-
 ?