Protein Variants | Comment | Organism |
---|---|---|
H12D | each of the mutant proteins H4A, H4C, M8L, H12L, H12D retains an intact dodecameric ring structure and no significant changes in catalytic and regulatory behavior are caused by the amino acid substitutions on the dodecamer surface. The substitution Ala4His completely abolishes the (NH4)2SO4-induced aggregation. However the H4C mutant protein precipitates nearly completely under the same salting out conditions | Escherichia coli |
H12L | each of the mutant proteins H4A, H4C, M8L, H12L, H12D retains an intact dodecameric ring structure and no significant changes in catalytic and regulatory behavior are caused by the amino acid substitutions on the dodecamer surface. The substitution Ala4His completely abolishes the (NH4)2SO4-induced aggregation. However the H4C mutant protein precipitates nearly completely under the same salting out conditions | Escherichia coli |
H4A | each of the mutant proteins H4A, H4C, M8L, H12L, H12D retains an intact dodecameric ring structure and no significant changes in catalytic and regulatory behavior are caused by the amino acid substitutions on the dodecamer surface. The substitution Ala4His completely abolishes the (NH4)2SO4-induced aggregation. However the H4C mutant protein precipitates nearly completely under the same salting out conditions | Escherichia coli |
H4C | each of the mutant proteins H4A, H4C, M8L, H12L, H12D retains an intact dodecameric ring structure and no significant changes in catalytic and regulatory behavior are caused by the amino acid substitutions on the dodecamer surface. The substitution Ala4His completely abolishes the (NH4)2SO4-induced aggregation. However the H4C mutant protein precipitates nearly completely under the same salting out conditions | Escherichia coli |
M8L | each of the mutant proteins H4A, H4C, M8L, H12L, H12D retains an intact dodecameric ring structure and no significant changes in catalytic and regulatory behavior are caused by the amino acid substitutions on the dodecamer surface. The substitution Ala4His completely abolishes the (NH4)2SO4-induced aggregation. However the H4C mutant protein precipitates nearly completely under the same salting out conditions | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and site-directed mutants H4A, H4C, M8L, H12L, H12D | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Escherichia coli | ADP + phosphate + L-Gln | - |
? |