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Literature summary for 6.3.1.2 extracted from

  • Kumar, S.; Nicholas, D.J.D.
    Purification, properties and regulation of glutamine synthetase from Nitrobacter agilis (1984), J. Gen. Microbiol., 130, 959-966.
No PubMed abstract available
Search for more literature for 6.3.1.2

Inhibitors

Inhibitors Comment Organism Structure
Ala
-
 Nitrobacter winogradskyi
Gly
-
 Nitrobacter winogradskyi
NH4Cl
-
 Nitrobacter winogradskyi
Ser
-
 Nitrobacter winogradskyi
Trp
-
 Nitrobacter winogradskyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 NH4+ biosynthetic assay Nitrobacter winogradskyi
2.6
-
 hydroxylamine glutamyltransferase reaction Nitrobacter winogradskyi
6.3
-
 Glu biosynthetic assay Nitrobacter winogradskyi
11.6
-
 Gln glutamyl transferase reaction Nitrobacter winogradskyi

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi
Cu2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi
Mg2+ divalent cation required, order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+, Cu2+, Ni2+ Nitrobacter winogradskyi
Mn2+ divalent cation required, order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+, Cu2+, Ni2+ Nitrobacter winogradskyi
Ni2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi
Zn2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 12 * 58000, SDS-PAGE Nitrobacter winogradskyi
700000
-
 gel filtration Nitrobacter winogradskyi

Organism

Organism UniProt Comment Textmining
Nitrobacter winogradskyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nitrobacter winogradskyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
-
 Nitrobacter winogradskyi

Storage Stability

Storage Stability Organism
-10°C, stable for several weeks Nitrobacter winogradskyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Nitrobacter winogradskyi ADP + phosphate + L-Gln
-
 ?
Gln + hydroxylamine + ADP
-
 Nitrobacter winogradskyi gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 58000, SDS-PAGE Nitrobacter winogradskyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Nitrobacter winogradskyi
7.8
-
 deadenylylated form Nitrobacter winogradskyi