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Literature summary for 6.3.1.2 extracted from
- Glutamine synthetase from muscle (1985), Methods Enzymol., 113, 199-212.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Ovis aries |  |
| ADP | - |
Rattus norvegicus | |
| ADP | - |
Sus scrofa | |
| Ala | D-Ala | Rattus norvegicus | |
| Ca2+ | above 2 mM CaCl2, in presence of 20 mM MgCl2 and 10 mM ATP | Rattus norvegicus | |
| Carbamoyl phosphate | - |
Rattus norvegicus | |
| D-Methionine sulfone | - |
Rattus norvegicus | |
| D-Methionine sulfone | - |
Sus scrofa | |
| diphosphate | - |
Rattus norvegicus | |
| GDP | weak | Ovis aries | |
| GDP | - |
Rattus norvegicus | |
| GDP | - |
Sus scrofa | |
| L-methionine sulfone | - |
Rattus norvegicus | |
| L-methionine sulfone | - |
Sus scrofa | |
| L-methionine sulfoximine | irreversible at high concentrations, competitive with L-Glu; S,R-sulfoximine and S-sulfoximine inhibit, R-sulfoximine is ineffective | Rattus norvegicus | |
| L-methionine sulfoximine | - |
Sus scrofa | |
| Mg2+ | inhibition at more than 20 mM excess of MgCl2 over ATP | Rattus norvegicus | |
| Mn2+ | above 2 mM MnCl2, in presence of 20 mM MgCl2 and 10 mM ATP | Rattus norvegicus | |
| phosphate | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | required | Sus scrofa | |
| Mg2+ | optimal ratio of MgCl2 to ATP is 2:1 at 1 mM ATP, inhibition at more than 20 mM excess of MgCl2 over ATP | Rattus norvegicus | |
| Mn2+ | optimal concentration is 2 mM, 25% of the maximal activity relative to Mg2+ activation | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| brain | - |
Sus scrofa | - |
| brain | - |
Ovis aries | - |
| heart | - |
Sus scrofa | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
- |
Sus scrofa |
| 1.1 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + hydroxylamine | - |
Ovis aries | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
| ATP + L-Glu + NH4+ | - |
Rattus norvegicus | ADP + phosphate + L-Gln | - |
? | |
| ATP + L-Glu + NH4+ | - |
Sus scrofa | ADP + phosphate + L-Gln | - |
? | |
| ATP + L-Glu + NH4+ | - |
Ovis aries | ADP + phosphate + L-Gln | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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