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Literature summary for 6.3.1.2 extracted from

  • Bhandari, B.; Nicholas, D.J.D.
    Properties of glutamine synthetase of bacteroids from root nodules of Glycine max (1986), Plant Sci., 44, 89-96.
No PubMed abstract available
Search for more literature for 6.3.1.2

Inhibitors

Inhibitors Comment Organism Structure
Ala
-
 Bradyrhizobium japonicum
ATP biosynthetic reaction Bradyrhizobium japonicum
CTP biosynthetic reaction Bradyrhizobium japonicum
Gly
-
 Bradyrhizobium japonicum
ITP biosynthetic reaction Bradyrhizobium japonicum
L-Glu competitive versus L-Gln and non-competitive versus hydroxylamine; gamma-glutamyl transferase activity Bradyrhizobium japonicum
NH4Cl glutamyl transferase reaction, competitive versus L-Gln and non-competitive versus hydroxylamine Bradyrhizobium japonicum
Ser
-
 Bradyrhizobium japonicum
UTP biosynthetic reaction Bradyrhizobium japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 NH4Cl second value 8.9 mM, biphasic plot Bradyrhizobium japonicum
3.3
-
 hydroxylamine
-
 Bradyrhizobium japonicum
11.1
-
 L-Gln glutamyl transferase reaction Bradyrhizobium japonicum
12.6
-
 L-Gln biosynthetic reaction Bradyrhizobium japonicum
14.3
-
 ATP
-
 Bradyrhizobium japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 12 * 63000, SDS-PAGE Bradyrhizobium japonicum
760000
-
 gel filtration Bradyrhizobium japonicum

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme I Bradyrhizobium japonicum

Source Tissue

Source Tissue Comment Organism Textmining
bacteroid of root nodules Bradyrhizobium japonicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Bradyrhizobium japonicum ADP + phosphate + L-Gln
-
 ?
ATP + L-glutamate + NH3 NH3 in form of NH4Cl Bradyrhizobium japonicum ADP + phosphate + L-glutamine
-
 ?
Gln + hydroxylamine + ADP
-
 Bradyrhizobium japonicum gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 63000, SDS-PAGE Bradyrhizobium japonicum