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Literature summary for 6.3.1.18 extracted from

  • Takeo, M.; Ohara, A.; Sakae, S.; Okamoto, Y.; Kitamura, C.; Kato, D.; Negoro, S.
    Function of a glutamine synthetase-like protein in bacterial aniline oxidation via gamma-glutamylanilide (2013), J. Bacteriol., 195, 4406-4414.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain SO58 Acinetobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 5 mM used in assay conditions Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
6 * 55000, SDS-PAGE Acinetobacter sp.
350000
-
gel filtration Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + aniline Acinetobacter sp. 100% activity ADP + phosphate + N5-phenyl-L-glutamine
-
?
ATP + L-glutamate + aniline Acinetobacter sp. YAA 100% activity ADP + phosphate + N5-phenyl-L-glutamine
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. YAA
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap Q Sepharose column chromatography and Hi-Trap butyl FF column chromatography Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + 2-chloroaniline 92% activity compared to aniline Acinetobacter sp. ?
-
?
ATP + L-glutamate + 2-chloroaniline 92% activity compared to aniline Acinetobacter sp. YAA ?
-
?
ATP + L-glutamate + 2-methylaniline 40% activity compared to aniline Acinetobacter sp. ?
-
?
ATP + L-glutamate + 2-methylaniline 40% activity compared to aniline Acinetobacter sp. YAA ?
-
?
ATP + L-glutamate + 3-chloroaniline 69% activity compared to aniline Acinetobacter sp. ?
-
?
ATP + L-glutamate + 3-chloroaniline 69% activity compared to aniline Acinetobacter sp. YAA ?
-
?
ATP + L-glutamate + 3-methylaniline 27% activity compared to aniline Acinetobacter sp. ?
-
?
ATP + L-glutamate + 3-methylaniline 27% activity compared to aniline Acinetobacter sp. YAA ?
-
?
ATP + L-glutamate + 4-chloroaniline 92% activity compared to aniline Acinetobacter sp. ?
-
?
ATP + L-glutamate + 4-methylaniline 45% activity compared to aniline Acinetobacter sp. ?
-
?
ATP + L-glutamate + aniline 100% activity Acinetobacter sp. ADP + phosphate + N5-phenyl-L-glutamine
-
?
ATP + L-glutamate + aniline 100% activity Acinetobacter sp. YAA ADP + phosphate + N5-phenyl-L-glutamine
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 55000, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
atdA1
-
Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10
-
Acinetobacter sp.