Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain SO58 | Acinetobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, 5 mM used in assay conditions | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
6 * 55000, SDS-PAGE | Acinetobacter sp. |
350000 | - |
gel filtration | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + aniline | Acinetobacter sp. | 100% activity | ADP + phosphate + N5-phenyl-L-glutamine | - |
? | |
ATP + L-glutamate + aniline | Acinetobacter sp. YAA | 100% activity | ADP + phosphate + N5-phenyl-L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. YAA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap Q Sepharose column chromatography and Hi-Trap butyl FF column chromatography | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + 2-chloroaniline | 92% activity compared to aniline | Acinetobacter sp. | ? | - |
? | |
ATP + L-glutamate + 2-chloroaniline | 92% activity compared to aniline | Acinetobacter sp. YAA | ? | - |
? | |
ATP + L-glutamate + 2-methylaniline | 40% activity compared to aniline | Acinetobacter sp. | ? | - |
? | |
ATP + L-glutamate + 2-methylaniline | 40% activity compared to aniline | Acinetobacter sp. YAA | ? | - |
? | |
ATP + L-glutamate + 3-chloroaniline | 69% activity compared to aniline | Acinetobacter sp. | ? | - |
? | |
ATP + L-glutamate + 3-chloroaniline | 69% activity compared to aniline | Acinetobacter sp. YAA | ? | - |
? | |
ATP + L-glutamate + 3-methylaniline | 27% activity compared to aniline | Acinetobacter sp. | ? | - |
? | |
ATP + L-glutamate + 3-methylaniline | 27% activity compared to aniline | Acinetobacter sp. YAA | ? | - |
? | |
ATP + L-glutamate + 4-chloroaniline | 92% activity compared to aniline | Acinetobacter sp. | ? | - |
? | |
ATP + L-glutamate + 4-methylaniline | 45% activity compared to aniline | Acinetobacter sp. | ? | - |
? | |
ATP + L-glutamate + aniline | 100% activity | Acinetobacter sp. | ADP + phosphate + N5-phenyl-L-glutamine | - |
? | |
ATP + L-glutamate + aniline | 100% activity | Acinetobacter sp. YAA | ADP + phosphate + N5-phenyl-L-glutamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 55000, SDS-PAGE | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
atdA1 | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Acinetobacter sp. |