Literature summary for 6.2.1.B5 extracted from

Mocibob, M.; Ivic, N.; Bilokapic, S.; Maier, T.; Luic, M.; Ban, N.; Weygand-Durasevic, I.
Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis (2010), Proc. Natl. Acad. Sci. USA, 107, 14585-14590.

Search for more literature for 6.2.1.B5

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0047	-	[acyl-carrier protein Atu2571]	pH 7.5, 30°C	Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25698	-	x * 25698, calculated from sequence	Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-alanine + [acyl-carrier protein Atu2571]	Agrobacterium tumefaciens	-	AMP + diphosphate + L-alanyl-[acyl-carrier protein Atu2571]	-	?

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	Q7CWR3	homologous to the catalytic domain of highly diverged, atypical seryl-tRNA synthetases found only in methanogenic archaea and are deprived of the tRNA-binding domain	-

Purification (Commentary)

Purification (Comment)	Organism
-	Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-alanine + [acyl-carrier protein Atu2571]	-	Agrobacterium tumefaciens	AMP + diphosphate + L-alanyl-[acyl-carrier protein Atu2571]	-	?
ATP + L-alanine + [acyl-carrier protein Atu2571]	the carrier protein Atu2571 is cloned and overexpressed in Escherichia coli (UniProt: A9CHM9). The 4'-phosphopantetheinyl prosthetic group is attached to purified apo-carrier proteins. The enzyme is homologous to the catalytic domain of highly diverged, atypical seryl-tRNA synthetases found only in methanogenic archaea and are deprived of the tRNA-binding domain. In comparison to seryl-tRNA synthetases, the homolog displays different and relaxed amino acid specificity. The homolog lacks canonical tRNA aminoacylating activity and instead transfers activated amino acid to phosphopantetheine prosthetic group of putative carrier proteins, whose genes are identified in the genomic surroundings of the homolog. In the ATP-diphosphate exchange assay the enzyme prefers L-alanine over L-glycine or L-serine about 30-40fold, and activates L-Glu and L-Pro albeit more than three orders of magnitude less efficiently than L-alanine	Agrobacterium tumefaciens	AMP + diphosphate + L-alanyl-[acyl-carrier protein Atu2571]	-	?

Subunits

Subunits	Comment	Organism
?	x * 25698, calculated from sequence	Agrobacterium tumefaciens

Synonyms

Synonyms	Comment	Organism
aa:CP ligase	-	Agrobacterium tumefaciens
Atu2573 protein	-	Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.31	-	[acyl-carrier protein Atu2571]	pH 7.5, 30°C	Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
68	-	[acyl-carrier protein Atu2571]	pH 7.5, 30°C	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)