General Stability | Organism |
---|---|
rapidly denatured when frozen and thawed one time | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | - |
Pseudomonas sp. | |
NEM | - |
Pseudomonas sp. | |
p-hydroxymercuribenzoate | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
290000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. MA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
4°C, stable for several weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-mercaptosuccinate + CoA | activity is 18% relative to L-malate | Pseudomonas sp. | ADP + phosphate + 2-mercaptosuccinyl-CoA | - |
? | |
ATP + 2-mercaptosuccinate + CoA | activity is 18% relative to L-malate | Pseudomonas sp. MA | ADP + phosphate + 2-mercaptosuccinyl-CoA | - |
? | |
ATP + DL-isocitrate + CoA | activity is 27% of the activity relative to L-malate | Pseudomonas sp. | ADP + phosphate + isocitryl-CoA | - |
? | |
ATP + DL-isocitrate + CoA | activity is 27% of the activity relative to L-malate | Pseudomonas sp. MA | ADP + phosphate + isocitryl-CoA | - |
? | |
ATP + malate + CoA | activity with D-malate is 28% relative to L-malate | Pseudomonas sp. | ADP + phosphate + malyl-CoA | - |
? | |
ATP + malate + CoA | activity with D-malate is 28% relative to L-malate | Pseudomonas sp. MA | ADP + phosphate + malyl-CoA | - |
? | |
ATP + succinate + CoA | activity is 105% relative to L-malate | Pseudomonas sp. | ADP + phosphate + succinyl-CoA | - |
? | |
ATP + succinate + CoA | activity is 105% relative to L-malate | Pseudomonas sp. MA | ADP + phosphate + succinyl-CoA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
70% loss of activity after 10 min | Pseudomonas sp. |