Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.8 extracted from

  • Foster, J.; Luo, B.; Nakata, P.A.
    An oxalyl-CoA dependent pathway of oxalate catabolism plays a role in regulating calcium oxalate crystal accumulation and defending against oxalate-secreting phytopathogens in Medicago truncatula (2016), PLoS ONE, 11, e0149850 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR(DE3) cells and Nicotiana tabacum leaves Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
oxalate at pH 8.0, temperature not specified in the publication Medicago truncatula

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Medicago truncatula 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + oxalate + CoA Medicago truncatula
-
AMP + diphosphate + oxalyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Medicago truncatula
-
ecotype R108
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephadex G-25 gel filtration Medicago truncatula

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oxalate + CoA
-
Medicago truncatula AMP + diphosphate + oxalyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Medicago truncatula

Synonyms

Synonyms Comment Organism
AAE3
-
Medicago truncatula
Oxalyl-CoA synthetase
-
Medicago truncatula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Medicago truncatula

Cofactor

Cofactor Comment Organism Structure
ATP
-
Medicago truncatula

General Information

General Information Comment Organism
malfunction reduction of enzyme expression increases susceptibility of Medicago truncatula to the oxalate-secreting fungal pathogen Sclerotinia sclerotiorum and results in the accumulation of druse crystals of calcium oxalate Medicago truncatula