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Literature summary for 6.2.1.8 extracted from

  • Foster, J.; Kim, H.U.; Nakata, P.A.; Browse, J.
    A previously unknown oxalyl-CoA synthetase is important for oxalate catabolism in Arabidopsis (2012), Plant Cell, 24, 1217-1229.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged enzyme in onion epidermal cell cytoplasm using the constitutive 35S promoter, and from binary construct, transfected into Agrobacterium tumefaciens, GFP-tagged enzyme is transiently expressed in Nicotiana benthamiana leaf cytoplasm Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information aae3-1 and aae3-2 are null mutants are deficient in oxalyl-CoA synthetase Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.149
-
 oxalate pH 8.0, temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Arabidopsis thaliana 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + oxalate + CoA Arabidopsis thaliana
-
 AMP + diphosphate + oxalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SMT7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oxalate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + oxalyl-CoA
-
 ?
ATP + oxalate + CoA specific for oxalate Arabidopsis thaliana AMP + diphosphate + oxalyl-CoA
-
 ?
additional information no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, glyoxylate, and glutarate Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
AAE3
-
 Arabidopsis thaliana
acyl-activating enzyme3
-
 Arabidopsis thaliana
Oxalyl-CoA synthetase
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity range, profile overview Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction aae3 null mutants show increased soluble and insoluble oxalate contents compared to the wild-type enzyme, loss of AAE3 interferes with seed development affecting the integrity and function of the seed coat, phenotype, overview Arabidopsis thaliana
metabolism the enzyme catalyzes the first step in oxalate degradation Arabidopsis thaliana
physiological function oxalyl-CoA synthetase is required for oxalate degradation, for normal seed development, and for defense against an oxalate-producing fungal pathogen, e.g. the fungus Sclerotinia sclerotiorum Arabidopsis thaliana